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@inproceedings{792495,
author = {Bouchal, Pavel and Hernychová, Lenka and Mudrochová, Monika and Brožková, Kristýna and Sobotková, Jarmila and Garbis, Spiros D. and Novák, Petr and Vyzula, Rostislav and Nenutil, Rudolf and Vojtěšek, Bořivoj},
address = {Siena, Italy},
booktitle = {8th Siena Meeting: From Genome To Proteome: Integration and Proteome Completion.},
keywords = {SELDI-TOF MS; biomarkers; protein isolation and identification; mass spectrometry},
language = {eng},
location = {Siena, Italy},
pages = {153-153},
publisher = {The University of Siena},
title = {The ways leading to identification of potential biomarkers in SELDI-TOF MS based proteomic studies},
year = {2008}
}
TY - JOUR
ID - 792495
AU - Bouchal, Pavel - Hernychová, Lenka - Mudrochová, Monika - Brožková, Kristýna - Sobotková, Jarmila - Garbis, Spiros D. - Novák, Petr - Vyzula, Rostislav - Nenutil, Rudolf - Vojtěšek, Bořivoj
PY - 2008
TI - The ways leading to identification of potential biomarkers in SELDI-TOF MS based proteomic studies
PB - The University of Siena
CY - Siena, Italy
KW - SELDI-TOF MS
KW - biomarkers
KW - protein isolation and identification
KW - mass spectrometry
N2 - SELDI-TOF MS is a protein profiling method often used in clinical proteomics for screening and biomarker discovery. Our effort in last year has been focused on identification of proteins of interest in projects based on SELDI analyses of rat plasma and breast tissue using high resolution mass spectrometry. Contributions of a panel of protein separation and/or identification method like tricine SDS-PAGE, 2-D PAGE, LC-MS/MS, FT-ICR, iTRAQ-2DLC-MS/MS for the biomarker identification in SELDI studies will be presented. As a result of these experiences, we have developed a method combining IMAC column preseparation, reverse phase LC, SELDI protein profiling, tricine SDS-PAGE, protein profile matching and MS/MS identification. Using this approach, we have identified several proteins potentially related to breast cancer development and cytostatic agens action.
ER -
BOUCHAL, Pavel, Lenka HERNYCHOVÁ, Monika MUDROCHOVÁ, Kristýna BROŽKOVÁ, Jarmila SOBOTKOVÁ, Spiros D. GARBIS, Petr NOVÁK, Rostislav VYZULA, Rudolf NENUTIL a Bořivoj VOJTĚŠEK. The ways leading to identification of potential biomarkers in SELDI-TOF MS based proteomic studies. In \textit{8th Siena Meeting: From Genome To Proteome: Integration and Proteome Completion.}. Siena, Italy: The University of Siena, 2008, s.~153.
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