2008
Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .
MATULOVÁ, PetraZákladní údaje
Originální název
Role of SRS2 and Mus81/MMS4 in processing recombination/replication intermediates .
Název česky
Role SRS2 a Mus81/MMS4 v procesu recombinace a replikace.
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Vydání
2008
Další údaje
Jazyk
angličtina
Typ výsledku
Audiovizuální tvorba
Obor
10600 1.6 Biological sciences
Stát vydavatele
Rakousko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Kód RIV
RIV/00216224:14310/08:00026760
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
DNA repair; homologous recombination; nuclease activity; MUS81; SRS2
Štítky
Příznaky
Mezinárodní význam
Změněno: 14. 11. 2008 16:24, RNDr. Petra Matulová, CSc.
V originále
Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific.
Česky
Homologous recombination (HR) is a process widespread in nature and essential to maintain the integrity of the genome via repair of DNA double-strand breaks (DSBs). Several models were proposed to understand the mechanism of double-strand break repair by HR, identify separate steps and role of the individual proteins involved. The Mus81/MMS4 complex is a DNA structure-specific endonuclease involved in replication fork stability and DNA repair. It seems from genetic experiments that Mus81/MMS4 could interact with Srs2, a helicase which on one hand prevents untimely recombination and on the other hand is necessary for certain pathways of homologous recombination. In our study, we confirmed the interaction between Mus81/MMS4 and Srs2. We then analyzed the stimulation of Mus81/MMS4 nuclease activity by Srs2 and we found out that this stimulation depends on the DNA subtrate involved in the reaction. Our experiments also indicate that the interaction between Mus81/MMS4 and Srs2 is specific.
Návaznosti
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| MSM0021622413, záměr |
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