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@proceedings{796096,
author = {Nejedlá, Petra and Norek, Adam and Žákovská, Alena},
booktitle = {VI International Conference on Ticks and Tick-borne Pathogens - Book of Proceedings - TTP-6 - The challenge of ticks in a warming planet},
keywords = {spirochetes; ticks; mosquitoes; DFM; PCR},
language = {eng},
title = {Borrelia burgdorferi sensu lato and other spirochetes in ticks and mosquitoes. From borreliae to unknown spirochetes.},
year = {2008}
}
TY - CONF
ID - 796096
AU - Nejedlá, Petra - Norek, Adam - Žákovská, Alena
PY - 2008
TI - Borrelia burgdorferi sensu lato and other spirochetes in ticks and mosquitoes. From borreliae to unknown spirochetes.
KW - spirochetes
KW - ticks
KW - mosquitoes
KW - DFM
KW - PCR
N2 - In our study we wanted to examine Ixodes ricinus ticks and different species of mosquitoes for the presence of pathogennic borreliae. We used two method: 1. Dark Field Microscopy (DFM) - detection of spirochetes 2. Polymerase Chain Reaction (PCR) - detection of Borrelia burgdorferi sensu lato DNA We found significantly higher positivity of ticks for borreliae compared to mosquitoes. There was the big discrepance in DFM and PCR positivity. It could be caused by PCR sensitivity (101 copies of DNA) and probably by the presence of other spirochetes. We isolated two bacteria strains from Culex (Culex) pipiens s.l., the first one was identified as B. afzelii, the second one identified as non B.b.s.l.
ER -
NEJEDLÁ, Petra, Adam NOREK and Alena ŽÁKOVSKÁ. Borrelia burgdorferi sensu lato and other spirochetes in ticks and mosquitoes. From borreliae to unknown spirochetes. In \textit{VI International Conference on Ticks and Tick-borne Pathogens - Book of Proceedings - TTP-6 - The challenge of ticks in a warming planet}. 2008.
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