Borrelia burgdorferi sensu lato in ectoparasites of small rodents - mites and fleas

NETUŠIL, Jakub, Alena ŽÁKOVSKÁ, Karel VOSTAL, Adam NOREK a Michal STANKO. Borrelia burgdorferi sensu lato in ectoparasites of small rodents - mites and fleas. In VI International Conference on Ticks and Tick-borne Pathogens - Book of Proceedings - TTP-6 - The challenge of ticks in a warming planet. 2008.

Základní údaje

• Originální název: Borrelia burgdorferi sensu lato in ectoparasites of small rodents - mites and fleas
• Název česky: Borrelia burgdorferi sensu lato v ektoparazitech malých hlodavců - roztočích a blechách
• Autoři: NETUŠIL, Jakub (203 Česká republika), Alena ŽÁKOVSKÁ (203 Česká republika, garant, domácí), Karel VOSTAL (203 Česká republika, domácí), Adam NOREK (203 Česká republika) a Michal STANKO (703 Slovensko).
• Vydání: VI International Conference on Ticks and Tick-borne Pathogens - Book of Proceedings - TTP-6 - The challenge of ticks in a warming planet, 2008.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Konferenční abstrakt
• Obor: Genetika a molekulární biologie
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/08:00026835
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova anglicky: B. burgdorferi s.l.; Acarina; Siphonaptera
• Štítky: Acarina, B. burgdorferi s.l., Siphonaptera
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

In our two year study ectoparasites of rodents were examined for the presence of borrelian DNA in order to find if there is a possibility of their participation in transfer of B.b.s.l within the vector-host cycle. The majority of parasites were members of the families Parasitidae and Dermanyssidae (Acarina) and families Hystrichopsyllidae and Ceratophyllidae (Siphonaptera). The most predominant species was Laelaps agilis, an obligate parasitic mite parasiting various hosts. In the first year the presence of spirochetes in the midgut of single parasites (only Acarina) was found out using Dark Field Microscopy (DFM). Each DFM-positive sample was further tested for the presence of borrelian DNA using Polymerase Chain Reaction (PCR) method, where the primers for flagelline were used (1 positive sample). In second year arthropods from Acarina and Siphonaptera groups were gathered and examined as individuals or pools of certain species just by PCR method. DNA of B.b.s.l. was detected in representatives of all examined groups of parasitic arthropods.

Anotace česky

In our two year study ectoparasites of rodents were examined for the presence of borrelian DNA in order to find if there is a possibility of their participation in transfer of B.b.s.l within the vector-host cycle. The majority of parasites were members of the families Parasitidae and Dermanyssidae (Acarina) and families Hystrichopsyllidae and Ceratophyllidae (Siphonaptera). The most predominant species was Laelaps agilis, an obligate parasitic mite parasiting various hosts. In the first year the presence of spirochetes in the midgut of single parasites (only Acarina) was found out using Dark Field Microscopy (DFM). Each DFM-positive sample was further tested for the presence of borrelian DNA using Polymerase Chain Reaction (PCR) method, where the primers for flagelline were used (1 positive sample). In second year arthropods from Acarina and Siphonaptera groups were gathered and examined as individuals or pools of certain species just by PCR method. DNA of B.b.s.l. was detected in representatives of all examined groups of parasitic arthropods.

Návaznosti

• MSM0021622415, záměr: Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací