2008
Borrelia burgdorferi sensu lato in ectoparasites of small rodents - mites and fleas
NETUŠIL, Jakub; Alena ŽÁKOVSKÁ; Karel VOSTAL; Adam NOREK; Michal STANKO et. al.Základní údaje
Originální název
Borrelia burgdorferi sensu lato in ectoparasites of small rodents - mites and fleas
Název česky
Borrelia burgdorferi sensu lato v ektoparazitech malých hlodavců - roztočích a blechách
Autoři
NETUŠIL, Jakub; Alena ŽÁKOVSKÁ; Karel VOSTAL; Adam NOREK a Michal STANKO
Vydání
VI International Conference on Ticks and Tick-borne Pathogens - Book of Proceedings - TTP-6 - The challenge of ticks in a warming planet, 2008
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/08:00026835
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
B. burgdorferi s.l.; Acarina; Siphonaptera
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 12. 1. 2012 20:41, doc. RNDr. Alena Žákovská, Ph.D.
V originále
In our two year study ectoparasites of rodents were examined for the presence of borrelian DNA in order to find if there is a possibility of their participation in transfer of B.b.s.l within the vector-host cycle. The majority of parasites were members of the families Parasitidae and Dermanyssidae (Acarina) and families Hystrichopsyllidae and Ceratophyllidae (Siphonaptera). The most predominant species was Laelaps agilis, an obligate parasitic mite parasiting various hosts. In the first year the presence of spirochetes in the midgut of single parasites (only Acarina) was found out using Dark Field Microscopy (DFM). Each DFM-positive sample was further tested for the presence of borrelian DNA using Polymerase Chain Reaction (PCR) method, where the primers for flagelline were used (1 positive sample). In second year arthropods from Acarina and Siphonaptera groups were gathered and examined as individuals or pools of certain species just by PCR method. DNA of B.b.s.l. was detected in representatives of all examined groups of parasitic arthropods.
Česky
In our two year study ectoparasites of rodents were examined for the presence of borrelian DNA in order to find if there is a possibility of their participation in transfer of B.b.s.l within the vector-host cycle. The majority of parasites were members of the families Parasitidae and Dermanyssidae (Acarina) and families Hystrichopsyllidae and Ceratophyllidae (Siphonaptera). The most predominant species was Laelaps agilis, an obligate parasitic mite parasiting various hosts. In the first year the presence of spirochetes in the midgut of single parasites (only Acarina) was found out using Dark Field Microscopy (DFM). Each DFM-positive sample was further tested for the presence of borrelian DNA using Polymerase Chain Reaction (PCR) method, where the primers for flagelline were used (1 positive sample). In second year arthropods from Acarina and Siphonaptera groups were gathered and examined as individuals or pools of certain species just by PCR method. DNA of B.b.s.l. was detected in representatives of all examined groups of parasitic arthropods.
Návaznosti
| MSM0021622415, záměr |
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