2005
Capillary zone electrophoresis of proteins and neuroprotective peptides
SPANILÁ, Miroslava; Jiří PAZOUREK; Dagmar GAJDOŠOVÁ; Ondřej ŠULÁK; Josef HAVEL et al.Základní údaje
Originální název
Capillary zone electrophoresis of proteins and neuroprotective peptides
Název česky
Kapilární zónová elektroforéza proteinů a neuroprotektivních peptidů
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Vydání
Geneva, Switzerland, Third International and Twenty-Eighth European Peptide Symposium. Peptides 2004. od s. 475-476, 2 s. 2005
Nakladatel
Kenes International
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/05:00025072
Organizační jednotka
Přírodovědecká fakulta
ISBN
965-90833-0-0
UT WoS
Klíčová slova anglicky
capillary coating; neuroprotective peptides; capillary zone electrophoresis
Změněno: 10. 12. 2008 15:55, Mgr. Miroslava Bittová, Ph.D.
V originále
Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of several background electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture of proteins (cytochrome C, lysozyme, ribonuclease A) and then applied to [Gly-14]-humanin.
Česky
Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of several background electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture of proteins (cytochrome C, lysozyme, ribonuclease A) and then applied to [Gly-14]-humanin.
Návaznosti
| GA305/03/1100, projekt VaV |
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