Další formáty:
BibTeX
LaTeX
RIS
@inproceedings{798481,
author = {Spanilá, Miroslava and Pazourek, Jiří and Gajdošová, Dagmar and Šulák, Ondřej and Havel, Josef},
address = {Geneva, Switzerland},
booktitle = {Third International and Twenty-Eighth European Peptide Symposium. Peptides 2004.},
keywords = {capillary coating; neuroprotective peptides; capillary zone electrophoresis},
language = {eng},
location = {Geneva, Switzerland},
isbn = {965-90833-0-0},
pages = {475-476},
publisher = {Kenes International},
title = {Capillary zone electrophoresis of proteins and neuroprotective peptides},
year = {2005}
}
TY - JOUR
ID - 798481
AU - Spanilá, Miroslava - Pazourek, Jiří - Gajdošová, Dagmar - Šulák, Ondřej - Havel, Josef
PY - 2005
TI - Capillary zone electrophoresis of proteins and neuroprotective peptides
PB - Kenes International
CY - Geneva, Switzerland
SN - 9659083300
KW - capillary coating
KW - neuroprotective peptides
KW - capillary zone electrophoresis
N2 - Biocompatibility of inner surface of capillaries in CE is critical for separation and detection of proteins and peptides. The problems come from adsorption of the peptides (proteins) on the surface because biomolecules are sensitive to changes in ionic strength, pH, and/or temperature of the electrolyte, etc. Therefore, modification of the fused silica capillary surface is often necessary. Procedures for capillary coating described in literature are usually complicated and time consuming. We optimized three types of relatively simple coating procedures suitable for protein and peptides separation: a/ polyethyleneimine (PEI), b/ 3-glycidoxypropyltrimethoxysilane (GPTMS), c/ 3-aminopropyltriethoxysilane (APS). Composition, concentration and pH of several background electrolyte were tested, namely citrate, phosphate, aminocaproate and TRIS. The capillary surface was characterized by determination of the electroosmotic flow (EOF). The conditions were optimized by running a standard mixture of proteins (cytochrome C, lysozyme, ribonuclease A) and then applied to [Gly-14]-humanin.
ER -
SPANILÁ, Miroslava, Jiří PAZOUREK, Dagmar GAJDOŠOVÁ, Ondřej ŠULÁK a Josef HAVEL. Capillary zone electrophoresis of proteins and neuroprotective peptides. In \textit{Third International and Twenty-Eighth European Peptide Symposium. Peptides 2004.}. Geneva, Switzerland: Kenes International, 2005, s.~475-476. ISBN~965-90833-0-0.
|
