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@article{815039,
author = {Svozilová, Zuzana and Kašparovský, Tomáš and Skládal, Petr and Lochman, Jan},
article_number = {2},
keywords = {Membrane receptor Membrane vesicles Real time bioanalysis Elicitins Kinetic},
language = {eng},
issn = {0003-2697},
journal = {Analytical biochemistry},
title = {Interaction of cryptogein with its binding sites in tobacco plasma},
volume = {390},
year = {2009}
}
TY - JOUR
ID - 815039
AU - Svozilová, Zuzana - Kašparovský, Tomáš - Skládal, Petr - Lochman, Jan
PY - 2009
TI - Interaction of cryptogein with its binding sites in tobacco plasma
JF - Analytical biochemistry
VL - 390
IS - 2
SP - 115-120
EP - 115-120
SN - 00032697
KW - Membrane receptor Membrane vesicles Real time bioanalysis Elicitins Kinetic
N2 - Elicitins are low molecular weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, this process is triggered by binding of elicitin to the high affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild type and mutant forms) were covalently immobilized on the sensing surface and membrane vesicles s containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association kas = 5.74x10E6 M1 s-1 and dissociation kdis = 6.87x10-4 s-1 constants, respectively. The kinetic equilibrium dissociation constant was calculated, KD = 12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.
ER -
SVOZILOVÁ, Zuzana, Tomáš KAŠPAROVSKÝ, Petr SKLÁDAL a Jan LOCHMAN. Interaction of cryptogein with its binding sites in tobacco plasma. \textit{Analytical biochemistry}. 2009, roč.~390, č.~2, s.~115-120. ISSN~0003-2697.
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