2009
Interaction of cryptogein with its binding sites in tobacco plasma
SVOZILOVÁ, Zuzana, Tomáš KAŠPAROVSKÝ, Petr SKLÁDAL a Jan LOCHMANZákladní údaje
Originální název
Interaction of cryptogein with its binding sites in tobacco plasma
Název česky
Interakce crytpogeinu s jeho vazabným místem na plazmatické membráně tabáku
Autoři
SVOZILOVÁ, Zuzana (203 Česká republika, domácí), Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí), Petr SKLÁDAL (203 Česká republika, domácí) a Jan LOCHMAN (203 Česká republika, garant, domácí)
Vydání
Analytical biochemistry, 2009, 0003-2697
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 3.287
Kód RIV
RIV/00216224:14310/09:00029172
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000266946000003
Klíčová slova anglicky
Membrane receptor Membrane vesicles Real time bioanalysis Elicitins Kinetic
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 8. 12. 2015 09:32, prof. Mgr. Tomáš Kašparovský, Ph.D.
V originále
Elicitins are low molecular weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, this process is triggered by binding of elicitin to the high affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild type and mutant forms) were covalently immobilized on the sensing surface and membrane vesicles s containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association kas = 5.74x10E6 M1 s-1 and dissociation kdis = 6.87x10-4 s-1 constants, respectively. The kinetic equilibrium dissociation constant was calculated, KD = 12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.
Česky
Elicitins are low molecular weight proteins representing the elicitor family secreted by many species of the oomycete Phytophthora. Elicitins induce a hypersensitive reaction in tobacco, this process is triggered by binding of elicitin to the high affinity site on the plasma membrane. Specific interaction of cryptogein with the binding sites on tobacco plasma membranes was studied using the piezoelectric biosensor in real time in a flow-through mode. Cryptogeins (wild type and mutant forms) were covalently immobilized on the sensing surface and membrane vesicles s containing receptors were in solution. Kinetic characterization of the interaction provided values of kinetic rate association kas = 5.74x10E6 M1 s-1 and dissociation kdis = 6.87x10-4 s-1 constants, respectively. The kinetic equilibrium dissociation constant was calculated, KD = 12.0 nM. The piezoelectric biosensor appeared to be a convenient tool for studying interactions of receptors embedded in membrane vesicles.
Návaznosti
| GA522/06/0156, projekt VaV |
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| MSM0021622413, záměr |
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