Auxin transport inhibitors impair vesicle motility and actin
cytoskeleton dynamics in diverse eukaryotes

J 2008

Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

DHONUKSHE, Pankaj; Ilya GRIGORIEV; Rainer FISCHER; Motoki TOMINAGA; David G ROBINSON et. al.

Základní údaje

Originální název

Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes

Název česky

Inhibitory auxinoveho transportu narusuji transport vacku a dynamiku aktinoveho cytoskeletu v ruznych eukaryotech.

Autoři

Vydání

Proceedings of the National Academy of Sciences of the United States of America, 2008, 0027-8424

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 9.380

Kód RIV

RIV/00216224:14310/08:00027622

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000254263300074

Klíčová slova anglicky

PIN proteins; plant development; vesicle traffic; auxin efflux inhibitors

Štítky

auxin efflux inhibitors, PIN proteins, plant development, vesicle traffic

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 2. 2010 17:17, Mgr. Gabriela Jalová

Anotace

ORIG CZ

V originále

Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.

Česky

Vyvoj rostlin do znacne miry zavisi na gradientech rostlineho hormonu auxinu. Inhibitory auxinoveho transportu (IAT), ktere narusuji auxinovy tok, byly nezbytne k formulaci tohoto konceptu, avsak mechanismus jejich ucinku neni znam. Zde ukazujeme, ze nektere IAT jako TIBA nebo PBA inhibuji transport vacku v rostlinach, kvasinkach a savcich bunkach. Efekty IAT na micropinocytozu, pohyb endosomu a pohyb fibroblastu indikuji, ze IAT ucinkuji na aktinovy cytoskelet. Techniky vizualizace aktinu ukazaly, ze IAT stabilizuji aktin v rostlinach, kvasinkach i savcich bunkach. Na druhou stranu, stabilizace aktinu pomoci genetickych nebo farmakologickych nastroju zpusobuje poruchy v endocytoze, transportu vacku, auxinovem transportu a vyvoji rostliny. Nase vysledky ukazuji, ze nektere IAT funguji jako stabilizatory aktinu a naznacuji, ze auxinovy transport vyzaduje transport vacku zavisly na aktinovem cytoskeletu. Tato studie je take prikladem jak pro ruzna eukaryota bezny a obecny proces transportu vacku muze mit specifickou roli v regulaci rostlineho vyvoje.

Návaznosti

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

DHONUKSHE, Pankaj; Ilya GRIGORIEV; Rainer FISCHER; Motoki TOMINAGA; David G ROBINSON; Jiří HAŠEK; Tomasz PACIOREK; Jan PETRÁŠEK; Daniela SEIFERTOVÁ; Ricardo TEJOS; Lee A. MEISELM; Eva ZAŽÍMALOVÁ; Theodorus W. J. GADELLA, JR; York-Dieter STIERHOF; Takashi UEDA; Kazuhiro OIWA; Anna AKHMANOVA; Roland BROCK; Anne SPANG a Jiří FRIML. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. Proceedings of the National Academy of Sciences of the United States of America. 2008, roč. 105, č. 11, s. 4489-4494. ISSN 0027-8424.

@article{816605,
   author = {Dhonukshe, Pankaj and Grigoriev, Ilya and Fischer, Rainer and Tominaga, Motoki and Robinson, David G and Hašek, Jiří and Paciorek, Tomasz and Petrášek, Jan and Seifertová, Daniela and Tejos, Ricardo and Meiselm, Lee A. and Zažímalová, Eva and Gadella, Jr, Theodorus W. J. and Stierhof, YorkandDieter and Ueda, Takashi and Oiwa, Kazuhiro and Akhmanova, Anna and Brock, Roland and Spang, Anne and Friml, Jiří},
   article_number = {11},
   keywords = {PIN proteins; plant development; vesicle traffic; auxin efflux inhibitors},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes},
   volume = {105},
   year = {2008}
}

TY  - JOUR
ID  - 816605
AU  - Dhonukshe, Pankaj - Grigoriev, Ilya - Fischer, Rainer - Tominaga, Motoki - Robinson, David G - Hašek, Jiří - Paciorek, Tomasz - Petrášek, Jan - Seifertová, Daniela - Tejos, Ricardo - Meiselm, Lee A. - Zažímalová, Eva - Gadella, Jr, Theodorus W. J. - Stierhof, York-Dieter - Ueda, Takashi - Oiwa, Kazuhiro - Akhmanova, Anna - Brock, Roland - Spang, Anne - Friml, Jiří
PY  - 2008
TI  - Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 105
IS  - 11
SP  - 4489-4494
EP  - 4489-4494
SN  - 00278424
KW  - PIN proteins
KW  - plant development
KW  - vesicle traffic
KW  - auxin efflux inhibitors
N2  - Many aspects of plant development, including patterning and tropisms, are largely dependent on the asymmetric distribution of the plant signaling molecule auxin. Auxin transport inhibitors (ATIs), which interfere with directional auxin transport, have been essential tools in formulating this concept. However, despite the use of ATIs in plant research for many decades, the mechanism of ATI action has remained largely elusive. Using real-time live-cell microscopy, we show here that prominent ATIs such as 2,3,5-triiodobenzoic acid (TIBA) and 2-(1-pyrenoyl) benzoic acid (PBA) inhibit vesicle trafficking in plant, yeast, and mammalian cells. Effects on micropinocytosis, rab5-labeled endosomal motility at the periphery of HeLa cells and on fibroblast mobility indicate that ATIs influence actin cytoskeleton. Visualization of actin cytoskeleton dynamics in plants, yeast, and mammalian cells show that ATIs stabilize actin. Conversely, stabilizing actin by chemical or genetic means interferes with endocytosis, vesicle motility, auxin transport, and plant development, including auxin transport-dependent processes. Our results show that a class of ATIs act as actin stabilizers and advocate that actin-dependent trafficking of auxin transport components participates in the mechanism of auxin transport. These studies also provide an example of how the common eukaryotic process of actin-based vesicle motility can fulfill a plant-specific physiological role.
ER  -

DHONUKSHE, Pankaj; Ilya GRIGORIEV; Rainer FISCHER; Motoki TOMINAGA; David G ROBINSON; Jiří HAŠEK; Tomasz PACIOREK; Jan PETRÁŠEK; Daniela SEIFERTOVÁ; Ricardo TEJOS; Lee A. MEISELM; Eva ZAŽÍMALOVÁ; Theodorus W. J. GADELLA, JR; York-Dieter STIERHOF; Takashi UEDA; Kazuhiro OIWA; Anna AKHMANOVA; Roland BROCK; Anne SPANG a Jiří FRIML. Auxin transport inhibitors impair vesicle motility and actin cytoskeleton dynamics in diverse eukaryotes. \textit{Proceedings of the National Academy of Sciences of the United States of America}. 2008, roč.~105, č.~11, s.~4489-4494. ISSN~0027-8424.

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