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@article{817461,
author = {Šmajs, David and Doležalová, Magda and Macek, Pavel and Žídek, Lukáš},
article_number = {21},
keywords = {colicin immunity; colicin Y; helix helix interaction; pore orming colicin; site drected mutagenesis},
language = {eng},
issn = {1742-464X},
journal = {FEBS Journal},
title = {Inactivation of colicin Y by intramembrane helix helix interaction with its immunity protein},
volume = {275},
year = {2008}
}
TY - JOUR
ID - 817461
AU - Šmajs, David - Doležalová, Magda - Macek, Pavel - Žídek, Lukáš
PY - 2008
TI - Inactivation of colicin Y by intramembrane helix helix interaction with its immunity protein
JF - FEBS Journal
VL - 275
IS - 21
SP - 5325 - 5331
EP - 5325 - 5331
SN - 1742464X
KW - colicin immunity
KW - colicin Y
KW - helix helix interaction
KW - pore orming colicin
KW - site drected mutagenesis
N2 - Construction of hybrids between colicins U and Y and mutagenesis of colicin Y gene (cya) revealed amino acid residues important for interactions between colicin Y and its cognate immunity protein (Cyi). Four such residues (I578, T582, Y586, and V590) were found in helices 8 and 9 of the colicin Y pore forming domain. To verify the importance of these residues, the corresponding amino acids in the colicin B protein were mutated to the residues present in colicin Y. An E. coli strain with cloned colicin Y immunity gene (cyi) inactivated this mutant but not the wildtype colicin B. In addition, interacting amino acid pairs in Cya and Cyi were identified using a set of Cyi point mutant strains. These data are consistent with antiparallel helix-helix interactions between Cyi helix T3 and Cya helix 8 of the pore-forming domain as a molecular mechanism of colicin Y inactivation by its immunity protein.
ER -
ŠMAJS, David, Magda DOLEŽALOVÁ, Pavel MACEK a Lukáš ŽÍDEK. Inactivation of colicin Y by intramembrane helix helix interaction with its immunity protein. Online. \textit{FEBS Journal}. 2008, roč.~275, č.~21, s.~5325 - 5331. ISSN~1742-464X. [citováno 2024-04-23]
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