Antioxidant lignans from petroleum ether extract of Schisandra
chinensis

J 2008

Antioxidant lignans from petroleum ether extract of Schisandra chinensis

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ a Otakar HUMPA

Základní údaje

Originální název

Antioxidant lignans from petroleum ether extract of Schisandra chinensis

Název česky

Antioxidační lignany z petroletherového extraktu Schisandra chinensis

Autoři

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ a Otakar HUMPA

Vydání

Planta Medica, Stuttgart - New York, Thieme Medical Publishers, 2008, 0032-0943

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.960

Organizační jednotka

Lékařská fakulta

UT WoS

000258261800209

Klíčová slova anglicky

Schisandra chinensis; lignans; DPPH; HPLC

Štítky

DPPH, HPLC, lignans, Schisandra chinensis

Příznaky

Mezinárodní význam

Změněno: 29. 11. 2011 16:09, Mgr. Michal Petr

Anotace

ORIG CZ

V originále

The fruit of Schisandra chinensis, a woody liana, has been used for centuries in traditional Chinese medicine. The fruit is prescribed for the treatment of viral and chemically induced hepatitis in China. Extracts of Schisandra chinensis have demonstrated hepatoprotective and cardioprotective activities, which can be potentially connected with ability to reduce oxidative stress. The aim of our study was to characterise compounds, which correspond for antioxidant activity of Schisandra chinensis. The petroleum ether extract of seeds of Schisandra chinensis showed scavenging activity against the free stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The petroleum ether extract was extracted with methanol and the methanolic extract was further chromatographed on silica gel column with petroleum ether, benzene, diethyl ether and ethanol as a mobile phase. The most active fractions eluted with benzene were further fractioned on silica gel column and Sephadex LH-20 column and by semi-preparative RP-HPLC. Bioassay guided fractionation led to the isolation of dibenzylbutane lignan meso-dihydroguaiaretic acid and dibenzo[a,c]cyclooctadiene lignans schisanhenol and gomisin J. All active lignans containing one or two phenolic hydroxyls belong to minor lignan constituents of Schisandra. Together with the active lignans, nine dibenzocyclooctadiene lignans were isolated, namely, schizandrin, deoxyschizandrin, gamma-schizandrin, gomisin A, gomisin N, tigloylgomisin H, angeloylgomisin H, tigloylgomisin P and wuweizisu C. The lignans were identified by spectroscopic methods (1H and 13C NMR, MS).

Česky

Antioxidační lignany, meso-dihydrogvajaretová kyselina,schisanhenol a gomisin J z petroletherového extraktu Schisandra chinensis byly vyizolovány.

Návaznosti

GA522/07/0995, projekt VaV

Název: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis

Investor: Grantová agentura ČR, Regulace biosyntézy sekundárních metabolitů v buněčné kultuře Schisandra chinensis

Citovat

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ a Otakar HUMPA. Antioxidant lignans from petroleum ether extract of Schisandra chinensis. Planta Medica. Stuttgart - New York: Thieme Medical Publishers, 2008, roč. 74, č. 9, s. 952-952. ISSN 0032-0943.

@article{818309,
   author = {Slanina, Jiří and Březinová, Lenka and Paulová, Hana and Humpa, Otakar},
   article_location = {Stuttgart - New York},
   article_number = {9},
   keywords = {Schisandra chinensis; lignans; DPPH; HPLC},
   language = {eng},
   issn = {0032-0943},
   journal = {Planta Medica},
   title = {Antioxidant lignans from petroleum ether extract of Schisandra chinensis},
   volume = {74},
   year = {2008}
}

TY  - JOUR
ID  - 818309
AU  - Slanina, Jiří - Březinová, Lenka - Paulová, Hana - Humpa, Otakar
PY  - 2008
TI  - Antioxidant lignans from petroleum ether extract of Schisandra chinensis
JF  - Planta Medica
VL  - 74
IS  - 9
SP  - 952-952
EP  - 952-952
PB  - Thieme Medical Publishers
SN  - 00320943
KW  - Schisandra chinensis
KW  - lignans
KW  - DPPH
KW  - HPLC
N2  - The fruit of Schisandra chinensis, a woody liana, has been used for centuries in traditional Chinese medicine. The fruit is prescribed for the treatment of viral and chemically induced hepatitis in China. Extracts of Schisandra chinensis have demonstrated hepatoprotective and cardioprotective activities, which can be potentially connected with ability to reduce oxidative stress. The aim of our study was to characterise compounds, which correspond for antioxidant activity of Schisandra chinensis. The petroleum ether extract of seeds of Schisandra chinensis showed scavenging activity against the free stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH. The petroleum ether extract was extracted with methanol and the methanolic extract was further chromatographed on silica gel column with petroleum ether, benzene, diethyl ether and ethanol as a mobile phase. The most active fractions eluted with benzene were further fractioned on silica gel column and Sephadex LH-20 column and by semi-preparative RP-HPLC. Bioassay guided fractionation led to the isolation of dibenzylbutane lignan meso-dihydroguaiaretic acid and dibenzo[a,c]cyclooctadiene lignans schisanhenol and gomisin J. All active lignans containing one or two phenolic hydroxyls belong to minor lignan constituents of Schisandra. Together with the active lignans, nine dibenzocyclooctadiene lignans were isolated, namely, schizandrin, deoxyschizandrin, gamma-schizandrin, gomisin A, gomisin N, tigloylgomisin H, angeloylgomisin H, tigloylgomisin P and wuweizisu C. The lignans were identified by spectroscopic methods (1H and 13C NMR, MS).
ER  -

SLANINA, Jiří, Lenka BŘEZINOVÁ, Hana PAULOVÁ a Otakar HUMPA. Antioxidant lignans from petroleum ether extract of Schisandra chinensis. \textit{Planta Medica}. Stuttgart - New York: Thieme Medical Publishers, 2008, roč.~74, č.~9, s.~952-952. ISSN~0032-0943.

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