a 2008

MICROCHIMERISM - LOOK INTO THE INCIPIENT AML RELAPSE?

HORKÝ, Ondřej, Jiří MAYER, Lenka KABLÁSKOVÁ, Marek BORSKÝ, Dana OHLÍDALOVÁ et. al.

Basic information

Original name

MICROCHIMERISM - LOOK INTO THE INCIPIENT AML RELAPSE?

Name in Czech

Mikrochimérizmus-vhled do počínajícího relapsu AML?

Authors

HORKÝ, Ondřej (203 Czech Republic, guarantor, belonging to the institution), Jiří MAYER (203 Czech Republic, belonging to the institution), Lenka KABLÁSKOVÁ (203 Czech Republic), Marek BORSKÝ (203 Czech Republic), Dana OHLÍDALOVÁ (203 Czech Republic), Marta KREJČÍ (203 Czech Republic, belonging to the institution) and Dana DVOŘÁKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Haematologica-the hematology journal, 2008

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30200 3.2 Clinical medicine

Country of publisher

Italy

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.978

RIV identification code

RIV/00216224:14110/08:00027839

Organization unit

Faculty of Medicine

ISSN

UT WoS

000263430201557

Keywords in English

mikrochimerism; relapse; prediction

Tags

International impact, Reviewed
Změněno: 9/5/2013 16:50, Mgr. Dana Hrabcová

Abstract

V originále

The reoccurrence or increase in autologous hematopoiesis after an allogeneic hematopoietic cell transplantation (allo-HCT) has been linked with incipient leukemia relapse, however, clear definition of such "emergency" in terms of microchimerism has not been defined yet. Aims Since the usual minimal residual disease (MRD) analysis is more sensitive than conventional chimerism analysis and MRD-guided intervention is routinely used after transplantation, here we wanted to explore if more sensitive chimerism analysis (microchimerism) could be used for earlier detection of imminent relapse. Methods We compared conventional polymerase chain reaction (PCR) of minisatellite, microsatellite, or sex specific regions with following fragment analysis (FA) (limit of quantification ~1%), with real-time quantitative PCR (RQ-PCR) of insertion/deletion and sex polymorphism (limit of quantification ~0.1%).

In Czech

Znovuobjevení se autologní krvetvorby po alogenní transplantaci hematopoetických kmenových buněk je spojováno s počínajícím relapsem základní choroby. Srovnání úspěšnosti predikce molekulárního relapsu na základě sledování minimální reziduální choroby a mikrochimérismu.

Links

MSM0021622430, plan (intention)
Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie
Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies