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@article{822895,
author = {Pohanka, Miroslav and Zbořil, Petr},
article_location = {Zagreb, Croatia},
article_number = {1},
keywords = {D-lactate; D-LCR; carbon paste electrode; amperometric biosensor},
issn = {1330-9862},
journal = {Food technology and biotechnology},
title = {Amperometric Biosensor for D-Lactate Assay},
volume = {46},
year = {2008}
}
TY - JOUR
ID - 822895
AU - Pohanka, Miroslav - Zbořil, Petr
PY - 2008
TI - Amperometric Biosensor for D-Lactate Assay
JF - Food technology and biotechnology
VL - 46
IS - 1
SP - 107-110
EP - 107-110
PB - UNIV ZAGREB
SN - 13309862
KW - D-lactate
KW - D-LCR
KW - carbon paste electrode
KW - amperometric biosensor
N2 - A carbon paste electrode biosensor for D-lactic acid analysis in biological samples was developed. D-lactate cytochrome c oxidoreductase from baker's yeast (EC 1.1.2.4) was used as biorecognition element of the electrode in combination with phenazine methosulphate (PMS)-Reinecke's salt precipitate as mediator. The enzyme was bound on paste due to Enzacryl using glutardialdehyde. The electrode response to D-lactate addition was linear in the concentration range of 0.015-100 mM. Limit of detection was equal to 56 mu M when signal vs. noise criterium (S/N=3) is considered. Specificity for only D-lactate was proven. Relative standard deviation of 6.6 % for 12 mM D-lactate was found. The biosensor exerts long-term stability and also good stability during repeated measuring cycles. The electrode has a good potential for use in practical samples.
ER -
POHANKA, Miroslav a Petr ZBOŘIL. Amperometric Biosensor for D-Lactate Assay. \textit{Food technology and biotechnology}. Zagreb, Croatia: UNIV ZAGREB, 2008, roč.~46, č.~1, s.~107-110. ISSN~1330-9862.
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