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@article{824758,
author = {Bártová, Eva and Harničarová, Andrea and Krejčí, Jana and Strašák, Luděk and Kozubek, Stanislav},
article_location = {Dordrecht},
article_number = {2},
keywords = {c-myc gene; nuclear speckles; nucleolus; promyelocytic leukaemia body;transcription site},
language = {eng},
issn = {0967-3849},
journal = {Chromosome Research},
title = {Single-cell c-myc gene expression in relationship to nuclear domains.},
volume = {16},
year = {2008}
}
TY - JOUR
ID - 824758
AU - Bártová, Eva - Harničarová, Andrea - Krejčí, Jana - Strašák, Luděk - Kozubek, Stanislav
PY - 2008
TI - Single-cell c-myc gene expression in relationship to nuclear domains.
JF - Chromosome Research
VL - 16
IS - 2
SP - 325-343
EP - 325-343
PB - Kluwer Academic Publishers
SN - 09673849
KW - c-myc gene
KW - nuclear speckles
KW - nucleolus
KW - promyelocytic leukaemia body;transcription site
N2 - Nuclear locations of the c-myc gene and its transcripts (c-myc (T)) have been investigated in relation to nuclear domains involved in RNA synthesis and processing. Transcription of the c-myc gene appears to be linked to the late G(1)- and preferentially to S-phases of the cell cycle. The c-myc gene and its transcripts were positioned non-randomly within the interphase nucleus; additionally, c-myc RNA signals accumulated at nucleoli. Using oligo-probes, designed to exon II and exon III of the c-myc gene, single c-myc (T) was preferentially observed in human carcinoma HT29 and A549 cells. Conversely, human embryonal teratocarcinoma NTERA cells were characterized by the presence of multiple c-myc RNA signals located in both the nucleoli and nucleoplasm. When accumulated at nucleoli, c-myc (T) occupied the periphery of this organelle, though not those associated with the cultivation surface. In HT29 cells, approximately 80% of c-myc (T) co-localized with the RNAP II positive regions, so-called transcription factories. However, in approximately 20% of the cells with c-myc transcripts, the c-myc (T) was released from the site of synthesis, and was not associated with either transcription factories or SC35 domains. In approximately 60% of nuclei with c-myc (T), these signals were located in close proximity to the SC35 regions, but promyelocytic leukaemia bodies were associated with c-myc (T) only in approximately 20% of the nuclei. Taken together, c-myc RNA signals were positioned in the most internal parts of the cell nuclei preferentially associated with the nucleoli. Specific nuclear and nucleolar positioning probably reflects the kinetics of c-myc RNA metabolism.
ER -
BÁRTOVÁ, Eva, Andrea HARNIČAROVÁ, Jana KREJČÍ, Luděk STRAŠÁK a Stanislav KOZUBEK. Single-cell c-myc gene expression in relationship to nuclear domains. \textit{Chromosome Research}. Dordrecht: Kluwer Academic Publishers, 2008, roč.~16, č.~2, s.~325-343. ISSN~0967-3849.
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