| HOFR, Ctirad, Pavla ŠULTESOVÁ, Michal ZIMMERMANN, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ, Michaela WIMMEROVÁ a Jiří FAJKUS. Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics. BIOCHEMICAL JOURNAL. ENGLAND: PORTLAND PRESS LTD, roč. 419, Neuveden, s. 221-228. ISSN 0264-6021. 2009. |
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@article{825540,
author = {Hofr, Ctirad and Šultesová, Pavla and Zimmermann, Michal and Mozgová, Iva and Procházková Schrumpfová, Petra and Wimmerová, Michaela and Fajkus, Jiří},
article_location = {ENGLAND},
article_number = {Neuveden},
keywords = {Fluorescence anisotropy; single-Myb-histone protein (SMH protein); surface plasmon resonance; telomere protein DNA interaction},
language = {eng},
issn = {0264-6021},
journal = {BIOCHEMICAL JOURNAL},
title = {Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics},
volume = {419},
year = {2009}
}
TY - JOUR
ID - 825540
AU - Hofr, Ctirad - Šultesová, Pavla - Zimmermann, Michal - Mozgová, Iva - Procházková Schrumpfová, Petra - Wimmerová, Michaela - Fajkus, Jiří
PY - 2009
TI - Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics
JF - BIOCHEMICAL JOURNAL
VL - 419
IS - Neuveden
SP - 221
EP - 221
PB - PORTLAND PRESS LTD
SN - 02646021
KW - Fluorescence anisotropy
KW - single-Myb-histone protein (SMH protein)
KW - surface plasmon resonance
KW - telomere protein DNA interaction
N2 - We performed the first quantitative DNA-binding study of the plant-specific family of SMH proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics. Based on these data, a model explaining the binding stoichiometry and the protein arrangement on telomeric DNA is presented.
ER -
HOFR, Ctirad, Pavla ŠULTESOVÁ, Michal ZIMMERMANN, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ, Michaela WIMMEROVÁ a Jiří FAJKUS. Single-Myb-histone proteins from Arabidopsis thaliana: a quantitative study of telomere-binding specifity and kinetics. \textit{BIOCHEMICAL JOURNAL}. ENGLAND: PORTLAND PRESS LTD, roč.~419, Neuveden, s.~221-228. ISSN~0264-6021. 2009.
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