Quantitative assessment of the effects of chemicals on
steroidogenic enzymes in the H295R cell line using real-time
quantitative PCR

J 2004

Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR

HILSCHEROVÁ, Klára; P.D. JONES; Tania GRACIA T; J.L. NEWSTED; X. ZHANG et. al.

Základní údaje

Originální název

Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR

Název česky

Kvantitativní hodnocení účinků chemických látek na steroidogenní enzymy v buněčné linii H295R pomocí real-time PCR

Autoři

HILSCHEROVÁ, Klára; P.D. JONES; Tania GRACIA T; J.L. NEWSTED; X. ZHANG; J.T. SANDERSON; R. YU; R. WU a J.P. GIESY

Vydání

Toxicological sciences, 2004, 1096-6080

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.391

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000223589100011

Klíčová slova česky

steroidogeneze; biotest; xenoestrogeny; skríning

Klíčová slova anglicky

Steroidogenesis; bioassay; xenoestrogens; screening

Štítky

bioassay, screening, steroidogenesis, xenoestrogens

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 8. 4. 2009 16:37, doc. Mgr. Klára Hilscherová, Ph.D.

Anotace

ORIG CZ

V originále

The potential for a variety of environmental contaminants to disturb endocrine function in wildlife species has been of recent concern. While much current effort is focused on the assessment of effects mediated through steroid hormone receptor based mechanisms, there is potentially a variety of other mechanisms, which could lead to endocrine disruption. Recent studies have demonstrated that a variety of xenobiotics can alter the gene expression or activity of enzymes involved in steroidogenesis. By altering the production of steroidogenic enzymes these chemicals have the potential to alter the delicate steroid balance in organisms. To assess the potential of chemicals to alter steroidogenesis an assay system was developed based on a human cell line that retains the ability to synthesize most of the important steroidogenic enzymes. Methods were developed to measure the expression of 10 genes involved in steroidogenesis by use of Q-RT-PCR. Assay conditions were optimized and expression levels were normalized to the expression of beta-actin. The effects of a variety of model chemicals known to alter steroid metabolism, both inducers and inhibitors, were assessed. Similar expression patterns were observed for chemicals acting through common mechanisms of action. Time-course studies demonstrated distinct time dependent expression profiles for chemicals able to modulate steroid metabolism. The assay, which allows simultaneous analysis of the expression of numerous steroidogenic enzymes, would be useful as a sensitive and rapid integrative screen for the many effects of chemicals on steroidogenesis.

Česky

Práce představuje nový model pro hodnocení ovlivnění steroidogeze xenobiotiky a metodu studia ovlivnění exprese steroidogeních enzymů pomocí real-time PCR.

Citovat

HILSCHEROVÁ, Klára; P.D. JONES; Tania GRACIA T; J.L. NEWSTED; X. ZHANG; J.T. SANDERSON; R. YU; R. WU a J.P. GIESY. Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR. Toxicological sciences. 2004, roč. 81, č. 1, s. 78 - 89. ISSN 1096-6080.

@article{827508,
   author = {Hilscherová, Klára and Jones, P.D. and Gracia T, Tania and Newsted, J.L. and Zhang, X. and Sanderson, J.T. and Yu, R. and Wu, R. and Giesy, J.P.},
   article_number = {1},
   keywords = {Steroidogenesis; bioassay; xenoestrogens; screening},
   language = {eng},
   issn = {1096-6080},
   journal = {Toxicological sciences},
   title = {Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR},
   volume = {81},
   year = {2004}
}

TY  - JOUR
ID  - 827508
AU  - Hilscherová, Klára - Jones, P.D. - Gracia T, Tania - Newsted, J.L. - Zhang, X. - Sanderson, J.T. - Yu, R. - Wu, R. - Giesy, J.P.
PY  - 2004
TI  - Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR
JF  - Toxicological sciences
VL  - 81
IS  - 1
SP  - 78 - 89
EP  - 78 - 89
SN  - 10966080
KW  - Steroidogenesis
KW  - bioassay
KW  - xenoestrogens
KW  - screening
N2  - The potential for a variety of environmental contaminants to disturb endocrine function in wildlife species has been of recent concern. While much current effort is focused on the assessment of effects mediated through steroid hormone receptor based mechanisms, there is potentially a variety of other mechanisms, which could lead to endocrine disruption. Recent studies have demonstrated that a variety of xenobiotics can alter the gene expression or activity of enzymes involved in steroidogenesis. By altering the production of steroidogenic enzymes these chemicals have the potential to alter the delicate steroid balance in organisms. To assess the potential of chemicals to alter steroidogenesis an assay system was developed based on a human cell line that retains the ability to synthesize most of the important steroidogenic enzymes. Methods were developed to measure the expression of 10 genes involved in steroidogenesis by use of Q-RT-PCR. Assay conditions were optimized and expression levels were normalized to the expression of beta-actin. The effects of a variety of model chemicals known to alter steroid metabolism, both inducers and inhibitors, were assessed. Similar expression patterns were observed for chemicals acting through common mechanisms of action. Time-course studies demonstrated distinct time dependent expression profiles for chemicals able to modulate steroid metabolism. The assay, which allows simultaneous analysis of the expression of numerous steroidogenic enzymes, would be useful as a sensitive and rapid integrative screen for the many effects of chemicals on steroidogenesis.
ER  -

HILSCHEROVÁ, Klára; P.D. JONES; Tania GRACIA T; J.L. NEWSTED; X. ZHANG; J.T. SANDERSON; R. YU; R. WU a J.P. GIESY. Quantitative assessment of the effects of chemicals on steroidogenic enzymes in the H295R cell line using real-time quantitative PCR. \textit{Toxicological sciences}. 2004, roč.~81, č.~1, s.~78 - 89. ISSN~1096-6080.

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