| KREJČÍ, Jana, Jiří PACHERNÍK, Aleš HAMPL a Petr DVOŘÁK. In vitro labelling of mouse embryonic stem cells with SPIO nanoparticles. General physiology and biophysics. Slovakia, 2008, roč. 27, č. 3, s. 164-173. ISSN 0231-5882. |
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@article{828361,
author = {Krejčí, Jana and Pacherník, Jiří and Hampl, Aleš and Dvořák, Petr},
article_location = {Slovakia},
article_number = {3},
keywords = {embryonic stem cells; SPIO nanoparticles; differentiation; magnetic labelling},
language = {eng},
issn = {0231-5882},
journal = {General physiology and biophysics},
title = {In vitro labelling of mouse embryonic stem cells with SPIO nanoparticles},
volume = {27},
year = {2008}
}
TY - JOUR
ID - 828361
AU - Krejčí, Jana - Pacherník, Jiří - Hampl, Aleš - Dvořák, Petr
PY - 2008
TI - In vitro labelling of mouse embryonic stem cells with SPIO nanoparticles
JF - General physiology and biophysics
VL - 27
IS - 3
SP - 164-173
EP - 164-173
SN - 02315882
KW - embryonic stem cells
KW - SPIO nanoparticles
KW - differentiation
KW - magnetic labelling
N2 - Labelling of mammalian cells with superparamagnetic iron oxide (SPIO) nanoparticles enables to monitor their fate in vivo using magnetic resonance imaging (MRI). However, the question remains whether or not SPIO nanoparticles affect the phenotype of labelled cells. In the present study, the effects of SPIO nanoparticles from two producers on the growth and differentiation of mouse embryonic stem (ES) cells in vitro were investigated. Our observations have shown that SPIO nanoparticles have no effect on the self-renewal of ES cells. Subsequently, we studied the effect of SPIO on the formation of embryoid bodies and neural differentiation of ES cell in monolayer culture. The cavitation of embryoid bodies was partially inhibited and neural differentiation was supported regardless the type of SPIO nanoparticles used. Thus for the first time we documented the effects of SPIO nanoparticles on ES cells and their differentiation.
ER -
KREJČÍ, Jana, Jiří PACHERNÍK, Aleš HAMPL a Petr DVOŘÁK. In vitro labelling of mouse embryonic stem cells with SPIO nanoparticles. \textit{General physiology and biophysics}. Slovakia, 2008, roč.~27, č.~3, s.~164-173. ISSN~0231-5882.
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