Biophysical Studies on the Stability of DNA Intrastrand
Cross-Links of Transplatin

J 2008

Biophysical Studies on the Stability of DNA Intrastrand Cross-Links of Transplatin

KAŠPÁRKOVÁ, Jana, María Victoria MARINI PALOMEQUE, Vendula BURSOVÁ a Viktor BRABEC

Základní údaje

Originální název

Biophysical Studies on the Stability of DNA Intrastrand Cross-Links of Transplatin

Název česky

Biofyzikální analýza stability vnitrořetězcových můstků transplatiny na DNA

Autoři

KAŠPÁRKOVÁ, Jana (203 Česká republika, garant, domácí), María Victoria MARINI PALOMEQUE (858 Uruguay, domácí), Vendula BURSOVÁ (203 Česká republika, domácí) a Viktor BRABEC (203 Česká republika, domácí)

Vydání

Biophysical Journal, USA, Biophysical Society, 2008, 0006-3495

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.683

Kód RIV

RIV/00216224:14310/08:00035832

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000260072600028

Klíčová slova anglicky

transplatin; DNA; antitumor

Štítky

antitumor, DNA, transplatin

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2011 20:46, Mgr. María Victoria Marini Palomeque, Ph.D.

Anotace

ORIG CZ

V originále

Clinically ineffective transplatin [trans-diamminedichloridoplatinum(II)] is used in the studies of the structure-pharmacological activity relationship of platinum compounds. In addition, a number of transplatin analogs exhibit promising toxic effects in several tumor cell lines including those resistant to conventional antitumor cisplatin. Moreover, transplatin-modified oligonucleotides have been shown to be effective modulators of gene expression. Owing to these facts and because DNA is also considered the major pharmacological target of platinum complexes, interactions between transplatin and DNA are of great interest. We examined, using biophysical and biochemical methods, the stability of 1,3-GNG intrastrand cross-links (CLs) formed by transplatin in short synthetic oligodeoxyribonucleotide duplexes and natural double-helical DNA. We have found that transplatin forms in double-helical DNA 1,3-GNG intrastrand CLs, but their stability depends on the sequence context. In some sequences the 1,3-GNG intrastrand CLs formed by transplatin in double-helical DNA readily rearrange into interstrand CLs. On the other hand, in a number of other sequences these intrastrand CLs are relatively stable. We show that the stability of 1,3-GNG intrastrand CLs of transplatin correlates with the extent of conformational distortion and thermodynamic destabilization induced in double-helical DNA by this adduct.

Česky

Klinicky neúčinná transplatina je předmětem studií vztahů struktury a farmakologické aktivity platinových komplexů.

Návaznosti

LC06030, projekt VaV

Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

KAŠPÁRKOVÁ, Jana, María Victoria MARINI PALOMEQUE, Vendula BURSOVÁ a Viktor BRABEC. Biophysical Studies on the Stability of DNA Intrastrand Cross-Links of Transplatin. Biophysical Journal. USA: Biophysical Society, 2008, roč. 95, č. 9, s. 4361-4371. ISSN 0006-3495.

@article{837278,
   author = {Kašpárková, Jana and Marini Palomeque, María Victoria and Bursová, Vendula and Brabec, Viktor},
   article_location = {USA},
   article_number = {9},
   keywords = {transplatin; DNA; antitumor},
   language = {eng},
   issn = {0006-3495},
   journal = {Biophysical Journal},
   title = {Biophysical Studies on the Stability of DNA Intrastrand Cross-Links of Transplatin},
   volume = {95},
   year = {2008}
}

TY  - JOUR
ID  - 837278
AU  - Kašpárková, Jana - Marini Palomeque, María Victoria - Bursová, Vendula - Brabec, Viktor
PY  - 2008
TI  - Biophysical Studies on the Stability of DNA Intrastrand Cross-Links of Transplatin
JF  - Biophysical Journal
VL  - 95
IS  - 9
SP  - 4361-4371
EP  - 4361-4371
PB  - Biophysical Society
SN  - 00063495
KW  - transplatin
KW  - DNA
KW  - antitumor
N2  - Clinically ineffective transplatin [trans-diamminedichloridoplatinum(II)] is used in the studies of the structure-pharmacological activity relationship of platinum compounds. In addition, a number of transplatin analogs exhibit promising toxic effects in several tumor cell lines including those resistant to conventional antitumor cisplatin. Moreover, transplatin-modified oligonucleotides have been shown to be effective modulators of gene expression. Owing to these facts and because DNA is also considered the major pharmacological target of platinum complexes, interactions between transplatin and DNA are of great interest. We examined, using biophysical and biochemical methods, the stability of 1,3-GNG intrastrand cross-links (CLs) formed by transplatin in short synthetic oligodeoxyribonucleotide duplexes and natural double-helical DNA. We have found that transplatin forms in double-helical DNA 1,3-GNG intrastrand CLs, but their stability depends on the sequence context. In some sequences the 1,3-GNG intrastrand CLs formed by transplatin in double-helical DNA readily rearrange into interstrand CLs. On the other hand, in a number of other sequences these intrastrand CLs are relatively stable. We show that the stability of 1,3-GNG intrastrand CLs of transplatin correlates with the extent of conformational distortion and thermodynamic destabilization induced in double-helical DNA by this adduct.
ER  -

KAŠPÁRKOVÁ, Jana, María Victoria MARINI PALOMEQUE, Vendula BURSOVÁ a Viktor BRABEC. Biophysical Studies on the Stability of DNA Intrastrand Cross-Links of Transplatin. \textit{Biophysical Journal}. USA: Biophysical Society, 2008, roč.~95, č.~9, s.~4361-4371. ISSN~0006-3495.

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