Binding of mismatch repair protein MutS to mispaired DNA
adducts of intercalating ruthenium(II) arene complexes

J 2008

Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes

CASTELLANO-CASTILLO, Maria, Hana KOSTRHUNOVA, María Victoria MARINI PALOMEQUE, Jana KAŠPÁRKOVÁ, Peter J. SADLER et. al.

Základní údaje

Originální název

Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes

Název česky

Vazba MutS na nezparovane DNA adukty interkalujicich rutheniovych komplexu

Autoři

CASTELLANO-CASTILLO, Maria (724 Španělsko), Hana KOSTRHUNOVA (203 Česká republika), María Victoria MARINI PALOMEQUE (858 Uruguay), Jana KAŠPÁRKOVÁ (203 Česká republika), Peter J. SADLER (826 Velká Británie a Severní Irsko), Jean-Marc MALINGE (250 Francie) a Viktor BRABEC (203 Česká republika, garant)

Vydání

Journal of Biological Inorganic Chemistry, Germany, Springer Berlin / Heidelberg, 2008, 0949-8257

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.600

Kód RIV

RIV/00216224:14310/08:00035834

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000257934500014

Klíčová slova česky

DNA ; Oprava nezparovanych bazi; MutS; rutheniove areny; interkalace

Klíčová slova anglicky

DNA ; Mismatch repair ; MutS ; Ruthenium arene ; Intercalation

Štítky

DNA, intercalation, MISMATCH REPAIR, MutS, Ruthenium arene

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 6. 2009 12:30, Mgr. María Victoria Marini Palomeque, Ph.D.

Anotace

ORIG CZ

V originále

The present study was performed to examine the affinity of Escherichia coli mismatch repair (MMR) protein MutS for DNA damaged by an intercalating compound. We examined the binding properties of this protein with various DNA substrates containing a single centrally located adduct of ruthenium(II) arene complexes [(eta(6)-arene)Ru(II)(en)Cl][PF(6)] [arene is tetrahydroanthracene (THA) or p-cymene (CYM); en is ethylenediamine]. These two complexes were chosen as representatives of two different classes of monofunctional ruthenium(II) arene compounds which differ in DNA-binding modes: one that involves combined coordination to G N7 along with noncovalent, hydrophobic interactions, such as partial arene intercalation (tricyclic-ring Ru-THA), and the other that binds to DNA only via coordination to G N7 and does not interact with double-helical DNA by intercalation (monoring Ru-CYM). Using electrophoretic mobility shift assays, we examined the binding properties of MutS protein with various DNA duplexes (homoduplexes or mismatched duplexes) containing a single centrally located adduct of ruthenium(II) arene compounds. We have shown that presence of the ruthenium(II) arene adducts decreases the affinity of MutS for ruthenated DNA duplexes that either have a regular sequence or contain a mismatch and that intercalation of the arene contributes considerably to this inhibitory effect. Since MutS initiates MMR by recognizing DNA lesions, the results of the present work support the view that DNA damage due to intercalation is removed from DNA by a mechanism(s) other than MMR.

Česky

Cíl práce byl studovat afinitu proteinu MutS na poškozenou DNA.

Návaznosti

LC06030, projekt VaV

Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

CASTELLANO-CASTILLO, Maria, Hana KOSTRHUNOVA, María Victoria MARINI PALOMEQUE, Jana KAŠPÁRKOVÁ, Peter J. SADLER, Jean-Marc MALINGE a Viktor BRABEC. Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes. Journal of Biological Inorganic Chemistry. Germany: Springer Berlin / Heidelberg, 2008, roč. 13, č. 6, s. 993-999. ISSN 0949-8257.

@article{837281,
   author = {CastellanoandCastillo, Maria and Kostrhunova, Hana and Marini Palomeque, María Victoria and Kašpárková, Jana and Sadler, Peter J. and Malinge, JeanandMarc and Brabec, Viktor},
   article_location = {Germany},
   article_number = {6},
   keywords = {DNA ; Mismatch repair ; MutS ; Ruthenium arene ; Intercalation},
   language = {eng},
   issn = {0949-8257},
   journal = {Journal of Biological Inorganic Chemistry},
   title = {Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes},
   volume = {13},
   year = {2008}
}

TY  - JOUR
ID  - 837281
AU  - Castellano-Castillo, Maria - Kostrhunova, Hana - Marini Palomeque, María Victoria - Kašpárková, Jana - Sadler, Peter J. - Malinge, Jean-Marc - Brabec, Viktor
PY  - 2008
TI  - Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes
JF  - Journal of Biological Inorganic Chemistry
VL  - 13
IS  - 6
SP  - 993-999
EP  - 993-999
PB  - Springer Berlin / Heidelberg
SN  - 09498257
KW  - DNA
KW  - Mismatch repair
KW  - MutS
KW  - Ruthenium arene
KW  - Intercalation
N2  - The present study was performed to examine the affinity of Escherichia coli mismatch repair (MMR) protein MutS for DNA damaged by an intercalating compound. We examined the binding properties of this protein with various DNA substrates containing a single centrally located adduct of ruthenium(II) arene complexes [(eta(6)-arene)Ru(II)(en)Cl][PF(6)] [arene is tetrahydroanthracene (THA) or p-cymene (CYM); en is ethylenediamine]. These two complexes were chosen as representatives of two different classes of monofunctional ruthenium(II) arene compounds which differ in DNA-binding modes: one that involves combined coordination to G N7 along with noncovalent, hydrophobic interactions, such as partial arene intercalation (tricyclic-ring Ru-THA), and the other that binds to DNA only via coordination to G N7 and does not interact with double-helical DNA by intercalation (monoring Ru-CYM). Using electrophoretic mobility shift assays, we examined the binding properties of MutS protein with various DNA duplexes (homoduplexes or mismatched duplexes) containing a single centrally located adduct of ruthenium(II) arene compounds. We have shown that presence of the ruthenium(II) arene adducts decreases the affinity of MutS for ruthenated DNA duplexes that either have a regular sequence or contain a mismatch and that intercalation of the arene contributes considerably to this inhibitory effect. Since MutS initiates MMR by recognizing DNA lesions, the results of the present work support the view that DNA damage due to intercalation is removed from DNA by a mechanism(s) other than MMR.
ER  -

CASTELLANO-CASTILLO, Maria, Hana KOSTRHUNOVA, María Victoria MARINI PALOMEQUE, Jana KAŠPÁRKOVÁ, Peter J. SADLER, Jean-Marc MALINGE a Viktor BRABEC. Binding of mismatch repair protein MutS to mispaired DNA adducts of intercalating ruthenium(II) arene complexes. \textit{Journal of Biological Inorganic Chemistry}. Germany: Springer Berlin / Heidelberg, 2008, roč.~13, č.~6, s.~993-999. ISSN~0949-8257.

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