Identification of chromosomal fusion sites in Arabidopsis
mutants using sequential bicolour BAC-FISH

J 2006

Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH

MOKROS, Petr

Základní údaje

Originální název

Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH

Název česky

Identifikace míst chromozomomálních fůzí u mutantů A. thaliana s použitím sekvenční bicolour BAC-FISH

Autoři

MOKROS, Petr

Vydání

Genome, NRC Research Press, 2006

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000242211800020

Klíčová slova česky

fůze chromosomů dvoubarevná FISH BAC proby atTERT

Klíčová slova anglicky

Chromosomal fusion bicolor FISH BAC probes atTERT

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 22. 6. 2009 13:31, Mgr. Jan Vrbský, Ph.D.

Anotace

ORIG CZ

V originále

Double stranded chromosomal breaks are repaired by homologous recombination or nonhomologous end joining (NHEJ). When broken chromosome ends are fused together by NHEJ, the resulting dicentric chromosomes can be detected as anaphase bridges during the subsequent mitosis. Telomeres in the absence of functional telomerase shorten, became unprotected, and are eventually recognized by the cell repair system as double stranded breaks. As result, chromosomes of Arabidopsis thaliana plants that are deficient in the gene for telomerase reverse transcriptase (TERT) are prone to chromosome fusions. We use Arabidopsis tert-/- mutants as a model system for analyzing terminal chromosome fusions. Here we report a novel and sensitive cytogenetic assay for the identification and characterization of chromosome-terminal fusion events by employing fluorescence in situ hybridization (FISH) with multiple probes and a repeated hybridization approach. A mixture of chromosome-specific subtelomeric probes is applied successively in 3 FISH reactions to the slides containing mitotic anaphase figures with anaphase bridges. Each figure is registered by a CCD camera after each in situ hybridization procedure. By comparing the signals presented on the bridge in successive images the assessment of the particular chromosome arms involved in fusion is possible. This experimental setup enables unambiguous identification of individual chromosome ends employed in fusion events.

Česky

Detekce dicentrických chromozomů tvořících anafázní mosty u TERT mutantů, kde jsou velice zkrácené teloméry a kde dochází s vysokou frekvencí k fúzi terminální chromozomů. Výsledkem této studie je navržení vhodných fluorescenčních prób, jejichž kombinacemi zle zjistit, které chromozómy a v jaké oblasti se na fúzi podílí.

Citovat

MOKROS, Petr. Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH. Genome. NRC Research Press, 2006, roč. 49, č. 8, s. 1036-1042.

@article{837710,
   author = {Mokros, Petr},
   article_number = {8},
   keywords = {Chromosomal fusion bicolor FISH BAC probes atTERT},
   language = {eng},
   journal = {Genome},
   title = {Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/17036078?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum},
   volume = {49},
   year = {2006}
}

TY  - JOUR
ID  - 837710
AU  - Mokros, Petr
PY  - 2006
TI  - Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH
JF  - Genome
VL  - 49
IS  - 8
SP  - 1036-1042
EP  - 1036-1042
PB  - NRC Research Press
KW  - Chromosomal fusion bicolor FISH BAC probes atTERT
UR  - http://www.ncbi.nlm.nih.gov/pubmed/17036078?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
L2  - http://www.ncbi.nlm.nih.gov/pubmed/17036078?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
N2  - Double stranded chromosomal breaks are repaired by homologous recombination or nonhomologous end joining (NHEJ). When broken chromosome ends are fused together by NHEJ, the resulting dicentric chromosomes can be detected as anaphase bridges during the subsequent mitosis. Telomeres in the absence of functional telomerase shorten, became unprotected, and are eventually recognized by the cell repair system as double stranded breaks. As result, chromosomes of Arabidopsis thaliana plants that are deficient in the gene for telomerase reverse transcriptase (TERT) are prone to chromosome fusions. We use Arabidopsis tert-/- mutants as a model system for analyzing terminal chromosome fusions. Here we report a novel and sensitive cytogenetic assay for the identification and characterization of chromosome-terminal fusion events by employing fluorescence in situ hybridization (FISH) with multiple probes and a repeated hybridization approach. A mixture of chromosome-specific subtelomeric probes is applied successively in 3 FISH reactions to the slides containing mitotic anaphase figures with anaphase bridges. Each figure is registered by a CCD camera after each in situ hybridization procedure. By comparing the signals presented on the bridge in successive images the assessment of the particular chromosome arms involved in fusion is possible. This experimental setup enables unambiguous identification of individual chromosome ends employed in fusion events.
ER  -

MOKROS, Petr. Identification of chromosomal fusion sites in Arabidopsis mutants using sequential bicolour BAC-FISH. \textit{Genome}. NRC Research Press, 2006, roč.~49, č.~8, s.~1036-1042.

Přehled o publikaci