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@proceedings{839238,
author = {Zimmermann, Michal and Hofr, Ctirad and Šultesová, Pavla and Mozgová, Iva and Procházková Schrumpfová, Petra and Fajkus, Jiří},
booktitle = {FluoroFest Prague 2009},
keywords = {Protein-DNA interaction; Arabidopsis thaliana; SMH protein; fluorescence anisotropy; electrophoretic mobility shift assay},
language = {eng},
title = {Application of fluorescence anisotropy to monitor protein binding to telomeric DNA},
year = {2009}
}
TY - CONF
ID - 839238
AU - Zimmermann, Michal - Hofr, Ctirad - Šultesová, Pavla - Mozgová, Iva - Procházková Schrumpfová, Petra - Fajkus, Jiří
PY - 2009
TI - Application of fluorescence anisotropy to monitor protein binding to telomeric DNA
KW - Protein-DNA interaction
KW - Arabidopsis thaliana
KW - SMH protein
KW - fluorescence anisotropy
KW - electrophoretic mobility shift assay
N2 - Using fluorescence anisotropy, we were able to compare the affinity of AtTRB proteins to fluorescently labeled DNA fragments harboring specific and nonspecific sequences and also to determine the optimal binding site by varying the size of the DNA fragments. Moreover, because fluorescence anisotropy enables the measurements to be performed in variety of experimental conditions, we managed to measure how the affinity depends on ionic strength. This allowed us to determine the electrostatic and nonelectrostatic contributions to the overall binding affinity of proteins to telomeric DNA. Based on these findings, a putative functional model of the complex between AtTRB proteins and telomeric DNA was constructed.
ER -
ZIMMERMANN, Michal, Ctirad HOFR, Pavla ŠULTESOVÁ, Iva MOZGOVÁ, Petra PROCHÁZKOVÁ SCHRUMPFOVÁ a Jiří FAJKUS. Application of fluorescence anisotropy to monitor protein binding to telomeric DNA. In \textit{FluoroFest Prague 2009}. 2009.
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