Continuous electrochemical monitoring of nitric oxide
production in murine macrophage cell line RAW 264.7

J 2009

Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7

PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK et. al.

Základní údaje

Originální název

Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7

Název česky

Měření produkce oxidu dusnatého u myších makrofágů RAW 264.7

Autoři

PEKAROVÁ, Michaela (703 Slovensko, garant), Jana MORAVCOVÁ (203 Česká republika), Lukáš KUBALA (203 Česká republika), Milan ČÍŽ (203 Česká republika), Antonín LOJEK (203 Česká republika), Čeněk GREGOR (203 Česká republika) a Jan HRBÁČ (203 Česká republika)

Vydání

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, HEIDELBERG, GERMANY, SPRINGER-VERLAG HEIDELBERG, 2009, 1618-2642

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.480

Kód RIV

RIV/00216224:14310/09:00028535

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000266918600029

Klíčová slova česky

oxid dusnatý, RAW 264.7, iNOS exprese, senzor pro oxid dusnatý

Klíčová slova anglicky

Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression

Štítky

iNOS expression, macrophages, nitric oxide, Nitric oxide sensor, Nitrites, RAW 264.7

Příznaky

Mezinárodní význam

Změněno: 26. 6. 2010 13:16, Mgr. Michaela Pekarová, Ph.D.

Anotace

ORIG CZ

V originále

In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment.

Česky

V této studii jsme se zaměřili na jednu z přímých metod stanovení produkce oxidu dusnatého v biologických systémech. Bylo realizováno dlouhodobé a kontinuální elektrochemické měření pomocí modifikovaného mikrosenzoru u makrofágů RAW 264.7.

Návaznosti

1QS500040507, projekt VaV

Název: Tukové složky výživy - modulace jejich účinků a možnosti praktického využití

Citovat

PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR a Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, roč. 394, č. 5, s. 1497-1504. ISSN 1618-2642.

@article{839617,
   author = {Pekarová, Michaela and Moravcová, Jana and Kubala, Lukáš and Číž, Milan and Lojek, Antonín and Gregor, Čeněk and Hrbáč, Jan},
   article_location = {HEIDELBERG, GERMANY},
   article_number = {5},
   keywords = {Nitric oxide; Macrophages; RAW 264.7; Nitric oxide sensor; Nitrites; iNOS expression},
   language = {eng},
   issn = {1618-2642},
   journal = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
   title = {Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7},
   volume = {394},
   year = {2009}
}

TY  - JOUR
ID  - 839617
AU  - Pekarová, Michaela - Moravcová, Jana - Kubala, Lukáš - Číž, Milan - Lojek, Antonín - Gregor, Čeněk - Hrbáč, Jan
PY  - 2009
TI  - Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7
JF  - ANALYTICAL AND BIOANALYTICAL CHEMISTRY
VL  - 394
IS  - 5
SP  - 1497-1504
EP  - 1497-1504
PB  - SPRINGER-VERLAG HEIDELBERG
SN  - 16182642
KW  - Nitric oxide
KW  - Macrophages
KW  - RAW 264.7
KW  - Nitric oxide sensor
KW  - Nitrites
KW  - iNOS expression
N2  - In this study, we realized the continual and long-term electrochemical detection of NO production by stimulated macrophages using modified porphyrinic micro-sensor. The NO release from RAW 264.7 cells stimulated by lipopolysaccharide started 5 h after the lipopolysaccharide administration. After reaching its maximum at the sixth hour, the stable level of NO production was observed between the seventh and 12th hour of the experiment. A close correlation between the NO signal detected with microelectrode and nitrite accumulation, which had been determined in supernatants removed from stimulated cells, was observed. The presence of iNOS enzyme, which constitutes a main requirement for NO production by stimulated macrophages, was confirmed by Western blot analysis of iNOS protein expression at key time points of the corresponding electrochemical experiment.
ER  -

PEKAROVÁ, Michaela, Jana MORAVCOVÁ, Lukáš KUBALA, Milan ČÍŽ, Antonín LOJEK, Čeněk GREGOR a Jan HRBÁČ. Continuous electrochemical monitoring of nitric oxide production in murine macrophage cell line RAW 264.7. \textit{ANALYTICAL AND BIOANALYTICAL CHEMISTRY}. HEIDELBERG, GERMANY: SPRINGER-VERLAG HEIDELBERG, 2009, roč.~394, č.~5, s.~1497-1504. ISSN~1618-2642.

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