Spliceosomal introns in a deep-branching eukaryote

VAŇÁČOVÁ, Štěpánka, Y. WEIHONG, Jane M. CARLTON a Patricia J. JOHNSON. Spliceosomal introns in a deep-branching eukaryote. Proceedings of the National Academy of Sciences of the U.S.A. 2005, roč. 102, č. 12, s. 4430-4435. ISSN 1091-6490.

Základní údaje

• Originální název: Spliceosomal introns in a deep-branching eukaryote
• Název česky: Spliceosomal introns in a deep-branching eukaryote
• Autoři: VAŇÁČOVÁ, Štěpánka (203 Česká republika, garant), Y. WEIHONG (156 Čína), Jane M. CARLTON (826 Velká Británie a Severní Irsko) a Patricia J. JOHNSON (840 Spojené státy).
• Vydání: Proceedings of the National Academy of Sciences of the U.S.A. 2005, 1091-6490.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: Genetika a molekulární biologie
• Stát vydavatele: Spojené státy
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/05:00036234
• Organizační jednotka: Přírodovědecká fakulta
• UT WoS: 000227854800039
• Klíčová slova česky: Trichomonas; pre-mRNA splicing; intron; evolution; deep branching eukaryote
• Klíčová slova anglicky: Trichomonas; pre-mRNA splicing; intron; evolution; deep branching eukaryote
• Štítky: deep branching eukaryote, Evolution, intron, pre-mRNA splicing, Trichomonas
• Příznaky: Mezinárodní význam, Recenzováno

Anotace

Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3-prime splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.

Anotace česky

Eukaryotes have evolved elaborate splicing mechanisms to remove introns that would otherwise destroy the protein-coding capacity of genes. Nuclear premRNA splicing requires sequence motifs in the intron and is mediated by a ribonucleoprotein complex, the spliceosome. Here we demonstrate the presence of a splicing apparatus in the protist Trichomonas vaginalis and show that RNA motifs found in yeast and metazoan introns are required for splicing. We also describe the first introns in this deep-branching lineage. The positions of these introns are often conserved in orthologous genes, indicating they were present in a common ancestor of trichomonads, yeast, and metazoa. All examined T. vaginalis introns have a highly conserved 12-nt 3-prime splice-site motif that encompasses the branch point and is necessary for splicing. This motif is also found in the only described intron in a gene from another deep-branching eukaryote, Giardia intestinalis. These studies demonstrate the conservation of intron splicing signals across large evolutionary distances, reveal unexpected motif conservation in deep-branching lineages that suggest a simplified mechanism of splicing in primitive unicellular eukaryotes, and support the presence of introns in the earliest eukaryote.