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@inproceedings{840518, author = {Fojta, Miroslav and Kostečka, Pavel and Bittová, Miroslava and Bartošík, Martin and Hianik, Tibor and Trnková, Libuše and Paleček, Emil}, address = {Ústí nad Labem}, booktitle = {XXIX. moderní elektrochemické metody - sborník příspěvků}, keywords = {DNA; OsO4 bipy; voltammetry; capillary electrophoresis}, howpublished = {tištěná verze "print"}, language = {eng}, location = {Ústí nad Labem}, isbn = {978-80-86238-39-5}, publisher = {BEST servis}, title = {Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes.}, year = {2009} }
TY - JOUR ID - 840518 AU - Fojta, Miroslav - Kostečka, Pavel - Bittová, Miroslava - Bartošík, Martin - Hianik, Tibor - Trnková, Libuše - Paleček, Emil PY - 2009 TI - Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. PB - BEST servis CY - Ústí nad Labem SN - 9788086238395 KW - DNA KW - OsO4 bipy KW - voltammetry KW - capillary electrophoresis N2 - In this work we used capillary zone electrophoresis (CZE) in combination with square wave voltammetry (SWV) at a pyrolytic graphite electrode (PGE) to study reactivity of different pyrimidine residues, thymine (T), uracil (U), cytosine (C) and 5 methyl cytosine (meC), within a pentanucleotide AAAAY (where Y stands for the pyrimidine residue), towards Os,bipy. Since adenine practically does not react with Os,L, Y residues represent the only reactive centre within the pentamers and their reactivity can thus be easily compared. Both techniques revealed the order of the Y reactivities as follows: T>>MeC~U>C. Hence, our results confirmed (a) highly preferential modification of T among all Ys and (b) increased reactivity of MeC, compared to C that was reported by other authors. However, despite recent papers presenting Os,L modification as useful tool for MeC detection in DNA, the strong modification of T within natural DNA (where T is much more abundant than MeC) makes this approach hardly applicable for real analyses of DNA methylation. ER -
FOJTA, Miroslav, Pavel KOSTEČKA, Miroslava BITTOVÁ, Martin BARTOŠÍK, Tibor HIANIK, Libuše TRNKOVÁ a Emil PALEČEK. Electrochemical and capillary zone electrophoresis study of pyrimidine residues modification by osmium tetroxide, 2,2´-bipyridine. Detection of unpaired and mispaired thymines in DNA heteroduplexes. In \textit{XXIX. moderní elektrochemické metody - sborník příspěvků}. Ústí nad Labem: BEST servis, 2009, 2 s. ISBN~978-80-86238-39-5.
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