Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.

VAŇÁČOVÁ, Štěpánka; Jan TACHEZY; Elisabetta ULLU a C. TSCHUDI. Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads. Molecular and Biochemical Parasitology. ELSEVIER, 2001, roč. 115, č. 2, s. 239-47, 9 s. ISSN 0166-6851.

Základní údaje

Originální název

Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.

Název česky

Unusual diversity in a-amanitin sensitivity of RNA polymerases in trichomonads.

Autoři

VAŇÁČOVÁ, Štěpánka; Jan TACHEZY; Elisabetta ULLU a C. TSCHUDI

Vydání

Molecular and Biochemical Parasitology, ELSEVIER, 2001, 0166-6851

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.397

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/01:00036247

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000169790600011

Klíčová slova česky

Trichomonads; RNA-dependent RNA polymerase; RNAP; amanitin

Klíčová slova anglicky

Trichomonads; RNA-dependent RNA polymerase; RNAP; amanitin

Štítky

amanitin, RNA-dependent RNA polymerase, RNAP, Trichomonads

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes.

Česky

Previous studies in the parasitic protist Trichomonas vaginalis have revealed that protein coding genes are transcribed by an alpha-amanitin-resistant RNA polymerase (RNAP) II. To investigate whether this unusual property is a general characteristic of trichomonads, we addressed the physiology of RNA synthesis in lysolecithin-permeabilized cells. Unlike in T. vaginalis, RNAP II in Tritrichomonas foetus was highly sensitive to the inhibitor alpha-amanitin. On the other hand, RNAP III, identified by its sensitivity to the specific inhibitor tagetitoxin, was found to be resistant to alpha-amanitin in Tritrichomonas foetus, but showed a typical intermediate sensitivity in T. vaginalis. Extension of this study to an additional seven trichomonad species confirmed this genera specific pattern of alpha-amanitin sensitivity and highlighted an unusual diversity in RNAPs among trichomonads, a closely related group of unicellular eukaryotes.