Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements

GUNZL, A.; Elisabetta ULLU; S.P. FRAGOSO; K.F. HOFFMANN; J.D. MILNER; Y. MORITA; E.K. NGUU; Štěpánka VAŇÁČOVÁ; S. WUNSCH; A.O. DARE; Helen KWON a C. TSCHUDI. Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements. Molecular and Biochemical Parasitology. ELSEVIER, 1997, roč. 85, č. 1, s. 67-76. ISSN 0166-6851.

Základní údaje

Originální název

Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements

Název česky

Transcription of the Trypanosoma brucei spliced leader RNA gene is dependent only on the presence of upstream regulatory elements

Autoři

GUNZL, A.; Elisabetta ULLU; S.P. FRAGOSO; K.F. HOFFMANN; J.D. MILNER; Y. MORITA; E.K. NGUU; Štěpánka VAŇÁČOVÁ; S. WUNSCH; A.O. DARE; Helen KWON a C. TSCHUDI

Vydání

Molecular and Biochemical Parasitology, ELSEVIER, 1997, 0166-6851

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Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.123

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/97:00036253

Organizační jednotka

Přírodovědecká fakulta

UT WoS

A1997WR49200006

Klíčová slova česky

Spliced leader RNA gene; In vitro transcription; Trypanosome; Trans-splicing; Trypanosoma brucei

Klíčová slova anglicky

Spliced leader RNA gene; In vitro transcription; Trypanosome; Trans-splicing; Trypanosoma brucei

Štítky

In vitro transcription, Spliced leader RNA gene, Trans-splicing, Trypanosoma brucei, Trypanosome

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

The spliced leader (SL) RNA plays a key role in mRNA maturation in trypanosomatid protozoa by providing the SL sequence, which is joined to the 5-prime end of every mRNA. As a first step towards a better understanding of the biogenesis and function of the SL RNA, we expressed a tagged SL RNA gene in a cell-free system of procyclic Trypanosoma brucei cells. Transcription initiates at +1 and can be detected as early as 1 min after addition of extract. Transcription of the SL RNA gene in vitro, as well as in permeable cells, is mediated by an alpha-amanitin/tagetitoxin resistant complex, suggesting a promoter that is intermediate between a classical RNA polymerase II and RNA polymerase III promoter. An analysis of the promoter architecture of the SL RNA gene revealed that regulatory elements are located upstream of the coding region and that the SL sequence, in contrast to the nematode SL sequence, is not required for T. brucei SL RNA gene transcription.

Česky

The spliced leader (SL) RNA plays a key role in mRNA maturation in trypanosomatid protozoa by providing the SL sequence, which is joined to the 5-prime end of every mRNA. As a first step towards a better understanding of the biogenesis and function of the SL RNA, we expressed a tagged SL RNA gene in a cell-free system of procyclic Trypanosoma brucei cells. Transcription initiates at +1 and can be detected as early as 1 min after addition of extract. Transcription of the SL RNA gene in vitro, as well as in permeable cells, is mediated by an alpha-amanitin/tagetitoxin resistant complex, suggesting a promoter that is intermediate between a classical RNA polymerase II and RNA polymerase III promoter. An analysis of the promoter architecture of the SL RNA gene revealed that regulatory elements are located upstream of the coding region and that the SL sequence, in contrast to the nematode SL sequence, is not required for T. brucei SL RNA gene transcription.