Bone marrow versus peripheral blood: comparison of the
chimerism analysis

a 2009

Bone marrow versus peripheral blood: comparison of the chimerism analysis

HORKÝ, Ondřej; Jiří MAYER; Lenka KABLÁSKOVÁ; Marta KREJČÍ; Yvona BRYCHTOVÁ et al.

Základní údaje

Originální název

Bone marrow versus peripheral blood: comparison of the chimerism analysis

Název česky

Kostní dřeň versus periferní krev: porovnání analýzy chimérismu

Autoři

HORKÝ, Ondřej; Jiří MAYER; Lenka KABLÁSKOVÁ; Marta KREJČÍ; Yvona BRYCHTOVÁ a Dana DVOŘÁKOVÁ

Vydání

14th Annual Meeting of the European Hematology Association, 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/09:00036469

Organizační jednotka

Lékařská fakulta

UT WoS

000266931901292

Klíčová slova česky

chimérismus; kostní dřeň; periferní krev

Klíčová slova anglicky

chimerism; bone marrow; peripheral blood

Změněno: 15. 2. 2010 06:46, Mgr. Ondřej Horký, Ph.D.

Anotace

ORIG CZ

V originále

Background Bone marrow (BM) examination is routinely performed for minimal residual disease monitoring in leukemia patients. By contrast, chimerism is usually tested from peripheral blood (PB), and, surprisingly, little is known about the clinical impact of the chimerism status in BM. Patients and Methods 226 pairs of PB and BM samples obtained from 102 adult patients were investigated. Frequencies of diagnoses within samples were as follows: AML 42%, CML 24%, ALL 11%, CLL 7%, MDS 7%, other hematological malignancies 9%. Assessment of chimerism was performed using either capillary electrophoresis with fluorescence detection, or quantitative real-time PCR with one order of magnitude higher sensitivity of mixed chimerism detection. Results Mixed chimerism in BM was detected even more than 9 years after transplantation, however, on the other hand, complete donor chimerism was also observed as early as day +35. Proportion of autologous cells detected in BM comparing to PB was: higher in 140/226 cases (62%), equal in 67 (30%), and smaller in 19 instances (8%). Within the group with higher autologous fraction in BM, 75/140 (54%) pairs were those with complete donor chimerism in PB and mixed chimerism in BM at the same time; the majority (61/75; 81%) of such BM samples contained only very small amount of autologous cells (bellow 0.1%). Nevertheless 18/140 cases (13%) possessed clear clinical relevance and all but one were from AML patients: chimerism detected in both compartments varied from 0.1% to 60% and corresponded to either impending relapse (n=16) or a graft failure (n=2). Conclusions Our data indicate that chimerism analysis in BM can provide new information in contrast to PB analysis in AML patients. Highly discrepant results with high autologous recovery in BM can be considered as a sign of imminent relapse or a graft failure. Two important questions are remaining to be further explored: what are the residual autologous cells causing the persisting mixed chimerism in the BM, and what is the best timing for BM chimerism analysis.

Česky

Návaznosti

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

HORKÝ, Ondřej; Jiří MAYER; Lenka KABLÁSKOVÁ; Marta KREJČÍ; Yvona BRYCHTOVÁ a Dana DVOŘÁKOVÁ. Bone marrow versus peripheral blood: comparison of the chimerism analysis. In 14th Annual Meeting of the European Hematology Association. 2009.

@proceedings{842469,
   author = {Horký, Ondřej and Mayer, Jiří and Kablásková, Lenka and Krejčí, Marta and Brychtová, Yvona and Dvořáková, Dana},
   booktitle = {14th Annual Meeting of the European Hematology Association},
   keywords = {chimerism; bone marrow; peripheral blood},
   language = {eng},
   title = {Bone marrow versus peripheral blood: comparison of the chimerism analysis},
   url = {http://www.eventure-online.com/eventure/publicAbstractView.do?id=101331},
   year = {2009}
}

TY  - CONF
ID  - 842469
AU  - Horký, Ondřej - Mayer, Jiří - Kablásková, Lenka - Krejčí, Marta - Brychtová, Yvona - Dvořáková, Dana
PY  - 2009
TI  - Bone marrow versus peripheral blood: comparison of the chimerism analysis
KW  - chimerism
KW  - bone marrow
KW  - peripheral blood
UR  - http://www.eventure-online.com/eventure/publicAbstractView.do?id=101331
N2  - Background Bone marrow (BM) examination is routinely performed for minimal residual disease monitoring in leukemia patients. By contrast, chimerism is usually tested from peripheral blood (PB), and, surprisingly, little is known about the clinical impact of the chimerism status in BM. Patients and Methods 226 pairs of PB and BM samples obtained from 102 adult patients were investigated. Frequencies of diagnoses within samples were as follows: AML 42%, CML 24%, ALL 11%, CLL 7%, MDS 7%, other hematological malignancies 9%. Assessment of chimerism was performed using either capillary electrophoresis with fluorescence detection, or quantitative real-time PCR with one order of magnitude higher sensitivity of mixed chimerism detection. Results Mixed chimerism in BM was detected even more than 9 years after transplantation, however, on the other hand, complete donor chimerism was also observed as early as day +35. Proportion of autologous cells detected in BM comparing to PB was: higher in 140/226 cases (62%), equal in 67 (30%), and smaller in 19 instances (8%). Within the group with higher autologous fraction in BM, 75/140 (54%) pairs were those with complete donor chimerism in PB and mixed chimerism in BM at the same time; the majority (61/75; 81%) of such BM samples contained only very small amount of autologous cells (bellow 0.1%). Nevertheless 18/140 cases (13%) possessed clear clinical relevance and all but one were from AML patients: chimerism detected in both compartments varied from 0.1% to 60% and corresponded to either impending relapse (n=16) or a graft failure (n=2). Conclusions Our data indicate that chimerism analysis in BM can provide new information in contrast to PB analysis in AML patients. Highly discrepant results with high autologous recovery in BM can be considered as a sign of imminent relapse or a graft failure. Two important questions are remaining to be further explored: what are the residual autologous cells causing the persisting mixed chimerism in the BM, and what is the best timing for BM chimerism analysis.
ER  -

HORKÝ, Ondřej; Jiří MAYER; Lenka KABLÁSKOVÁ; Marta KREJČÍ; Yvona BRYCHTOVÁ a Dana DVOŘÁKOVÁ. Bone marrow versus peripheral blood: comparison of the chimerism analysis. In \textit{14th Annual Meeting of the European Hematology Association}. 2009.

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