NOVÁKOVÁ, Olga, Jaroslav MALINA, Jana KAŠPÁRKOVÁ, Anna HALÁMIKOVÁ, Vladan BERNARD, Francesco INTINI, Giovanni NATILE a Viktor BRABEC. Energetics, conformation and recognition of DNA duplexes modified by methylated analougues of [PtCl(dien)]+. Chemistry – A European Journal. Germany: Wiley, 2009, roč. 2009, č. 15, s. 6211-6221. ISSN 0947-6539.
Další formáty:   BibTeX LaTeX RIS
Základní údaje
Originální název Energetics, conformation and recognition of DNA duplexes modified by methylated analougues of [PtCl(dien)]+
Autoři NOVÁKOVÁ, Olga, Jaroslav MALINA, Jana KAŠPÁRKOVÁ, Anna HALÁMIKOVÁ, Vladan BERNARD, Francesco INTINI, Giovanni NATILE a Viktor BRABEC.
Vydání Chemistry – A European Journal, Germany, Wiley, 2009, 0947-6539.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10610 Biophysics
Stát vydavatele Německo
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 5.382
Organizační jednotka Přírodovědecká fakulta
UT WoS 000267501400018
Klíčová slova česky DNA, DNA struktury, platina, polymerizace
Klíčová slova anglicky DNA, DNA recognition, DNA structures, platinum, polymerization
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: doc. Mgr. Vladan Bernard, Ph.D., učo 60122. Změněno: 13. 1. 2011 11:39.
Anotace
In early studies of empirical structure–activity relationships, monodentate PtII complexes were considered to be biologically inactive. Examples of such inactive monodentate PtII compounds are [PtCl (dien)]+ dien=diethylenetriamine) and [PtCl(NH3)3]+. DNA is considered the major biological target of platinum compounds. Thus, monodentate DNA binding of PtII compounds was previously expected to display insignificant biological effects because it was assumed to affect DNA conformation and downstream cellular processes markedly less than the cross-links of bifunctional PtII complexes. More recently it was shown that some monodentate PtII complexes do exhibit biological effects; the active monodentate PtII complexes commonly feature bulkier amine ligands than the hitherto used dien or NH3 groups. We were therefore interested in determining whether a simple but marked enhancement of the bulkiness of the dien ligand in monodentate [Pt (NO3)- (dien)]+ by multiple methylation of this ligand affects the early phases in which platinum compounds exert their biological activity. More specifically, the goals of this study, performed in cell-free media, were to determine how the modification of DNA duplexes by methylated analogues of [Pt (NO3)- (dien)]+ affects their energetics and how the alterations of this biophysical parameter are reflected by the recognition of these duplexes by DNA polymerases and the DNA repair system. We have found that the impact of the methylation of [Pt (NO3) (dien)]+ on the biophysical properties of DNA (thermodynamic, thermal, and conformational properties) and its biochemical processes (DNA polymerization and the repair of DNA adducts) is remarkable. Hence, we conclude that monodentate DNA binding of PtII compounds may considerably affect the biophysical properties of DNA and consequently downstream cellular processes as a result of a large increase in the bulkiness of the nonleaving ligands in this class of metal complex.
VytisknoutZobrazeno: 18. 7. 2024 16:38