2009
Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
BENEŠOVÁ, Jana; Pavel DOBEŠ a Pavel HYRŠLZákladní údaje
Originální název
Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
Název česky
Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
Autoři
Vydání
Bulletin of Insectology, Bologna, 2009, 1721-8861
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30105 Physiology
Stát vydavatele
Itálie
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.448
Kód RIV
RIV/00216224:14310/09:00036599
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000272023300017
Klíčová slova česky
Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol.
Klíčová slova anglicky
Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol.
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 30. 1. 2016 23:11, Mgr. Pavel Dobeš, Ph.D.
V originále
Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. PO activity changed gradually during development except during larval-pupal and pupal-adult metamorphosis, where marked increases in activity occurred. Comparable changes with slight time shifts were determined in cuticle after its homogenization. Intact cuticles showed only minimal PO activity without distinct developmental changes. Experiments using pyrogallol as substrate confirmed the temporary decrease of overall PO activity during pupal stage of development and the subsequent return to high PO levels in adults. Developmental changes in phenol-oxidizing activity are independent of sex in both pupal and adult stage of G. mellonella.
Česky
Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. PO activity changed gradually during development except during larval-pupal and pupal-adult metamorphosis, where marked increases in activity occurred. Comparable changes with slight time shifts were determined in cuticle after its homogenization. Intact cuticles showed only minimal PO activity without distinct developmental changes. Experiments using pyrogallol as substrate confirmed the temporary decrease of overall PO activity during pupal stage of development and the subsequent return to high PO levels in adults. Developmental changes in phenol-oxidizing activity are independent of sex in both pupal and adult stage of G. mellonella.