BENEŠOVÁ, Jana, Pavel DOBEŠ a Pavel HYRŠL. Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L. Bulletin of Insectology, Bologna, 2009, roč. 62, č. 2, s. 237-243. ISSN 1721-8861.
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Základní údaje
Originální název Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
Název česky Developmental changes in phenol-oxidizing activity in the greater wax moth Galleria mellonella L.
Autoři BENEŠOVÁ, Jana (203 Česká republika, domácí), Pavel DOBEŠ (203 Česká republika) a Pavel HYRŠL (203 Česká republika, garant, domácí).
Vydání Bulletin of Insectology, Bologna, 2009, 1721-8861.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor Physiology
Stát vydavatele Itálie
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 0.448
Kód RIV RIV/00216224:14310/09:00036599
Organizační jednotka Přírodovědecká fakulta
UT WoS 000272023300017
Klíčová slova česky Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol.
Klíčová slova anglicky Development; Galleria mellonella; Hydroquinone; Laccase; Phenol-oxidazing activity; Phenoloxidase; Pyrogallol.
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: Mgr. Pavel Dobeš, Ph.D., učo 150960. Změněno: 30. 1. 2016 23:11.
Anotace
Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. PO activity changed gradually during development except during larval-pupal and pupal-adult metamorphosis, where marked increases in activity occurred. Comparable changes with slight time shifts were determined in cuticle after its homogenization. Intact cuticles showed only minimal PO activity without distinct developmental changes. Experiments using pyrogallol as substrate confirmed the temporary decrease of overall PO activity during pupal stage of development and the subsequent return to high PO levels in adults. Developmental changes in phenol-oxidizing activity are independent of sex in both pupal and adult stage of G. mellonella.
Anotace česky
Activity of enzymes oxidizing phenolic substrates commonly termed phenoloxidases (POs) was investigated during normal development of Galleria mellonella in haemolymph, intact cuticle and homogenized cuticle. PO activities were determined colorimetrically following chemical activation of prophenoloxidase with acetone or methanol using hydroquinone and pyrogallol as substrates. Hydroquinone with para-positioned hydroxyl group seems to be most suitable for spectrophotometrical detection of phenol-oxidizing activity in various insect tissues because its oxidation products interact only minimally with tissue material. Enzymatic activity changed markedly during development in larvae, pupae and adults. The highest levels of PO in haemolymph were detected using hydroquinone on the fifth day of the last larval instar and at the beginning and at the end of pupal stage. PO activity changed gradually during development except during larval-pupal and pupal-adult metamorphosis, where marked increases in activity occurred. Comparable changes with slight time shifts were determined in cuticle after its homogenization. Intact cuticles showed only minimal PO activity without distinct developmental changes. Experiments using pyrogallol as substrate confirmed the temporary decrease of overall PO activity during pupal stage of development and the subsequent return to high PO levels in adults. Developmental changes in phenol-oxidizing activity are independent of sex in both pupal and adult stage of G. mellonella.
VytisknoutZobrazeno: 24. 8. 2019 05:19