miRNA expression profile of regulatory T cells in patiens with
diabetes mellitus type I

a 2009

miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I

HÉŽOVÁ, Renata; Ondřej SLABÝ; Petra FALTEJSKOVÁ; Zuzana MIKULKOVÁ; Ivana BUREŠOVÁ et. al.

Základní údaje

Originální název

miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I

Název česky

miRNA expresní profil regulačních T lymfocytů u pacientů s diabetes mellitus typu I

Autoři

HÉŽOVÁ, Renata; Ondřej SLABÝ; Petra FALTEJSKOVÁ; Zuzana MIKULKOVÁ; Ivana BUREŠOVÁ; J. HODEK; J. OVESNÁ a Jaroslav MICHÁLEK

Vydání

The 4th Asian Congress on Autoimmunity, Singapore, 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Singapur

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

diabetes; regulační T lymfocyty; miRNA

Klíčová slova anglicky

Diabetes mellitus; regulatory T cells; miRNA

Změněno: 5. 9. 2012 16:18, Mgr. Petra Vychytilová, Ph.D.

Anotace

ORIG CZ

V originále

MicroRNA (miRNA) are small non-coding RNA molecules, that have a potential to regulate the expression of many genes. Type I diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic beta cells by cytotoxic T cells. Regulatory T cells (Treg) are specialised lymphocytes preventing from development of autoimmune disorders. Study on transgenic mouse with conditional Dicer knockout Treg revealed that this Treg cell-specific miRNA ablation results in the loss of suppressor function and development of fatal systemic autoimmune disease in vivo. Therefore we can assume, that the functional defect of Treg in T1D patients can be caused by different expression miRNA profile in these cells. The aim of our study was to establish expression profile of miRNA in patients with T1D and compare it with healthy donors. Six diabetic patients and 6 healthy volunteers provided peripheral blood for flow cytometry analyses and separation of Treg (CD3+, CD4+, CD25+, CD127-) and non-Treg cells (CD3+, CD4+, CD25-). Total RNA rich in small RNAs was isolated and used for TaqMan Human MicroRNA Array that analysed expression of 384 miRNA in Treg and non-Treg cells. The data were subsequently processed by HCL, SAM analysis. Different miRNA expression patterns were identified in Treg and non-Treg populations as well as in Treg cells between T1D and healthy individuals. We aimed to identify miRNA specific in pathogenesis of diabetes mellitus which could be used in the future as therapeutic targets of diabetic patients.

Česky

Cílem prezentované studie bylo získat expresní profil miRNA u pacientů s T1D a porovnat jej s profilem zdravých dárců.

Návaznosti

NR9355, projekt VaV

Název: Imunoregulační funkce u dětských pacientů s diabetes mellitus

2B08066, projekt VaV

Název: Studium léčebných možností diabetes mellitus 1. typu jako geneticky determinované metabolické choroby s využitím nových imunoterapeutických postupů

Citovat

HÉŽOVÁ, Renata; Ondřej SLABÝ; Petra FALTEJSKOVÁ; Zuzana MIKULKOVÁ; Ivana BUREŠOVÁ; J. HODEK; J. OVESNÁ a Jaroslav MICHÁLEK. miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I. In The 4th Asian Congress on Autoimmunity, Singapore. 2009.

@proceedings{846779,
   author = {Héžová, Renata and Slabý, Ondřej and Faltejsková, Petra and Mikulková, Zuzana and Burešová, Ivana and Hodek, J. and Ovesná, J. and Michálek, Jaroslav},
   booktitle = {The 4th Asian Congress on Autoimmunity, Singapore},
   keywords = {Diabetes mellitus; regulatory T cells; miRNA},
   language = {eng},
   title = {miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I},
   year = {2009}
}

TY  - CONF
ID  - 846779
AU  - Héžová, Renata - Slabý, Ondřej - Faltejsková, Petra - Mikulková, Zuzana - Burešová, Ivana - Hodek, J. - Ovesná, J. - Michálek, Jaroslav
PY  - 2009
TI  - miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I
KW  - Diabetes mellitus
KW  - regulatory T cells
KW  - miRNA
N2  - MicroRNA (miRNA) are small non-coding RNA molecules, that have a potential to regulate the expression of many genes. Type I diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic beta cells by cytotoxic T cells. Regulatory T cells (Treg) are specialised lymphocytes preventing from development of autoimmune disorders. Study on transgenic mouse with conditional Dicer knockout Treg revealed that this Treg cell-specific miRNA ablation results in the loss of suppressor function and development of fatal systemic autoimmune disease in vivo. Therefore we can assume, that the functional defect of Treg in T1D patients can be caused by different expression miRNA profile in these cells. The aim of our study was to establish expression profile of miRNA in patients with T1D and compare it with healthy donors. Six diabetic patients and 6 healthy volunteers provided peripheral blood for flow cytometry analyses and separation of Treg (CD3+, CD4+, CD25+, CD127-) and non-Treg cells (CD3+, CD4+, CD25-). Total RNA rich in small RNAs was isolated and used for TaqMan Human MicroRNA Array that analysed expression of 384 miRNA in Treg and non-Treg cells. The data were subsequently processed by HCL, SAM analysis. Different miRNA expression patterns were identified in Treg and non-Treg populations as well as in Treg cells between T1D and healthy individuals. We aimed to identify miRNA specific in pathogenesis of diabetes mellitus which could be used in the future as therapeutic targets of diabetic patients.
ER  -

HÉŽOVÁ, Renata; Ondřej SLABÝ; Petra FALTEJSKOVÁ; Zuzana MIKULKOVÁ; Ivana BUREŠOVÁ; J. HODEK; J. OVESNÁ a Jaroslav MICHÁLEK. miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I. In \textit{The 4th Asian Congress on Autoimmunity, Singapore}. 2009.

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