5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin
D3 induced monocytic differentiation by activating p38 MAPK
pathway

J 2009

5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway

STIXOVÁ, Lenka; Jiřina PROCHÁZKOVÁ; Karel SOUČEK; Jiřina HOFMANOVÁ; Alois KOZUBÍK et. al.

Základní údaje

Originální název

5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway

Autoři

STIXOVÁ, Lenka (203 Česká republika, garant); Jiřina PROCHÁZKOVÁ (203 Česká republika); Karel SOUČEK (203 Česká republika); Jiřina HOFMANOVÁ (203 Česká republika) a Alois KOZUBÍK (203 Česká republika)

Vydání

Mol Cell Biochem, 2009, 0300-8177

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.896

Kód RIV

RIV/00216224:14310/09:00028603

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000270687500025

Klíčová slova anglicky

Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2010 11:43, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation.

Citovat

STIXOVÁ, Lenka; Jiřina PROCHÁZKOVÁ; Karel SOUČEK; Jiřina HOFMANOVÁ a Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. Mol Cell Biochem. 2009, roč. 330, 1-2, 10 s. ISSN 0300-8177.

@article{849022,
   author = {Stixová, Lenka and Procházková, Jiřina and Souček, Karel and Hofmanová, Jiřina and Kozubík, Alois},
   article_number = {1-2},
   keywords = {Differentiation; 1alpha25 dihydroxyvitamin D3; 5 lipoxygenase inhibitors; p38 MAPK;},
   language = {eng},
   issn = {0300-8177},
   journal = {Mol Cell Biochem},
   title = {5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway},
   volume = {330},
   year = {2009}
}

TY  - JOUR
ID  - 849022
AU  - Stixová, Lenka - Procházková, Jiřina - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois
PY  - 2009
TI  - 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway
JF  - Mol Cell Biochem
VL  - 330
IS  - 1-2
SN  - 03008177
KW  - Differentiation
KW  - 1alpha25 dihydroxyvitamin D3
KW  - 5 lipoxygenase inhibitors
KW  - p38 MAPK;
N2  - The treatment of human promyelocytic leukemia cell lines HL-60, and to some extent NB-4, with 1alpha,25-dihydroxyvitamin D3 (VD3) induces differentiation towards the monocytic/macrophage lineage, demonstrated by the increased expression of CD11b and CD14, and the production of opsonized zymosan particles (OZP) stimulated reactive oxygen species (ROS). Moreover, in more sensitive HL-60 cells, increased expression of 5-lipoxygenase (5-LPO), Mcl-1, IkappaB and c-Jun, accompanied by the activation of p38 MAPK, was detected. These VD3 effects on HL-60 cell differentiation were significantly potentiated by 5-LPO inhibitors MK-886 and AA-861 and were inverted by SB202190 (SB), a p38 MAPK inhibitor. The inhibition of differentiation by SB was demonstrated by a reduction of CD14 expression and by a decrease in OZP activated ROS production. These results indicated that p38 MAPK pathway is involved in 5-LPO inhibitors-dependent potentiation of VD3-induced monocytic differentiation.
ER  -

STIXOVÁ, Lenka; Jiřina PROCHÁZKOVÁ; Karel SOUČEK; Jiřina HOFMANOVÁ a Alois KOZUBÍK. 5 lipoxygenase inhibitors potentiate 1alpha,25 dihydroxyvitamin D3 induced monocytic differentiation by activating p38 MAPK pathway. \textit{Mol Cell Biochem}. 2009, roč.~330, 1-2, 10 s. ISSN~0300-8177.

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