Monocytic differentiation of leukemic HL-60 cells induced by
co-treatment with TNF-alpha and MK886 requires activation of
pro-apoptotic machinery

J 2009

Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery

PROCHÁZKOVÁ, Jiřina, Lenka STIXOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ, Alois KOZUBÍK et. al.

Základní údaje

Originální název

Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery

Autoři

PROCHÁZKOVÁ, Jiřina (203 Česká republika, garant), Lenka STIXOVÁ (203 Česká republika), Karel SOUČEK (203 Česká republika), Jiřina HOFMANOVÁ (203 Česká republika) a Alois KOZUBÍK (203 Česká republika)

Vydání

European Journal of Haematology, 2009, 0902-4441

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Dánsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.345

Kód RIV

RIV/00216224:14310/09:00028604

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000266875000004

Klíčová slova anglicky

caspases; MAPK; MK886; NFkappaB; TNF-alpha

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2010 11:28, Mgr. Jiřina Medalová, Ph.D.

Anotace

V originále

Acute myeloid leukemia cells can be cured by differentiating therapy, but it has side effects. A study of other differentiation signaling pathways is therefore useful. We demonstrated previously that the co-treatment of HL-60 cells with Tumor necrosis factor-a (TNF-a) (1 ng/mL) and inhibitor of 5-lipoxygenase MK886 (5 lM) potentiated both monocytic differentiation and apoptosis. In this study, we detected enhanced activation of three main types of MAPKs (p38, JNK, ERK). The inhibition of pro-apoptotic MAPKs (p38 and JNK) suppressed the effect of MK886 + TNF-a co-treatment. On the other hand, down-regulation of prosurvival ERK pathway led to increased differentiation. Those effects were accompanied by increased activation of caspases in cells treated by MK886 + TNF-a. Pan-caspase inhibitor ZVAD-fmk significantly decreased both number of apoptotic and differentiated cells. The same effect was observed after inhibition of caspase 9, but not caspase 3 and 8.

Citovat

PROCHÁZKOVÁ, Jiřina, Lenka STIXOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ a Alois KOZUBÍK. Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery. European Journal of Haematology. 2009, roč. 83, February, 13 s. ISSN 0902-4441.

@article{849023,
   author = {Procházková, Jiřina and Stixová, Lenka and Souček, Karel and Hofmanová, Jiřina and Kozubík, Alois},
   article_number = {February},
   keywords = {caspases; MAPK; MK886; NFkappaB; TNF-alpha},
   language = {eng},
   issn = {0902-4441},
   journal = {European Journal of Haematology},
   title = {Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery},
   volume = {83},
   year = {2009}
}

TY  - JOUR
ID  - 849023
AU  - Procházková, Jiřina - Stixová, Lenka - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois
PY  - 2009
TI  - Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery
JF  - European Journal of Haematology
VL  - 83
IS  - February
SN  - 09024441
KW  - caspases
KW  - MAPK
KW  - MK886
KW  - NFkappaB
KW  - TNF-alpha
N2  - Acute myeloid leukemia cells can be cured by differentiating therapy, but it has side effects. A study of other differentiation signaling pathways is therefore useful. We demonstrated previously that the co-treatment of HL-60 cells with Tumor necrosis factor-a (TNF-a) (1 ng/mL) and inhibitor of 5-lipoxygenase MK886 (5 lM) potentiated both monocytic differentiation and apoptosis. In this study, we detected enhanced activation of three main types of MAPKs (p38, JNK, ERK). The inhibition of pro-apoptotic MAPKs (p38 and JNK) suppressed the effect of MK886 + TNF-a co-treatment. On the other hand, down-regulation of prosurvival ERK pathway led to increased differentiation. Those effects were accompanied by increased activation of caspases in cells treated by MK886 + TNF-a. Pan-caspase inhibitor ZVAD-fmk significantly decreased both number of apoptotic and differentiated cells. The same effect was observed after inhibition of caspase 9, but not caspase 3 and 8.
ER  -

PROCHÁZKOVÁ, Jiřina, Lenka STIXOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ a Alois KOZUBÍK. Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery. \textit{European Journal of Haematology}. 2009, roč.~83, February, 13 s. ISSN~0902-4441.

Podrobný výpis o publikaci