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@article{849023,
author = {Procházková, Jiřina and Stixová, Lenka and Souček, Karel and Hofmanová, Jiřina and Kozubík, Alois},
article_number = {February},
keywords = {caspases; MAPK; MK886; NFkappaB; TNF-alpha},
language = {eng},
issn = {0902-4441},
journal = {European Journal of Haematology},
title = {Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery},
volume = {83},
year = {2009}
}
TY - JOUR
ID - 849023
AU - Procházková, Jiřina - Stixová, Lenka - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois
PY - 2009
TI - Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery
JF - European Journal of Haematology
VL - 83
IS - February
SN - 09024441
KW - caspases
KW - MAPK
KW - MK886
KW - NFkappaB
KW - TNF-alpha
N2 - Acute myeloid leukemia cells can be cured by differentiating therapy, but it has side effects. A study of other differentiation signaling pathways is therefore useful. We demonstrated previously that the co-treatment of HL-60 cells with Tumor necrosis factor-a (TNF-a) (1 ng/mL) and inhibitor of 5-lipoxygenase MK886 (5 lM) potentiated both monocytic differentiation and apoptosis. In this study, we detected enhanced activation of three main types of MAPKs (p38, JNK, ERK). The inhibition of pro-apoptotic MAPKs (p38 and JNK) suppressed the effect of MK886 + TNF-a co-treatment. On the other hand, down-regulation of prosurvival ERK pathway led to increased differentiation. Those effects were accompanied by increased activation of caspases in cells treated by MK886 + TNF-a. Pan-caspase inhibitor ZVAD-fmk significantly decreased both number of apoptotic and differentiated cells. The same effect was observed after inhibition of caspase 9, but not caspase 3 and 8.
ER -
PROCHÁZKOVÁ, Jiřina, Lenka STIXOVÁ, Karel SOUČEK, Jiřina HOFMANOVÁ a Alois KOZUBÍK. Monocytic differentiation of leukemic HL-60 cells induced by co-treatment with TNF-alpha and MK886 requires activation of pro-apoptotic machinery. \textit{European Journal of Haematology}. 2009, roč.~83, February, 13 s. ISSN~0902-4441.
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