2009
The Histidine Kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 Regulate Vascular Tissue Development in Arabidopsis Shoots
HEJÁTKO, Jan, Hojin RYU, Gyung-Tae KIM, Romana DOBEŠOVÁ, Sunhwa CHOI et. al.Základní údaje
Originální název
The Histidine Kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 Regulate Vascular Tissue Development in Arabidopsis Shoots
Autoři
HEJÁTKO, Jan (203 Česká republika, garant), Hojin RYU (410 Korejská republika), Gyung-Tae KIM (410 Korejská republika), Romana DOBEŠOVÁ (203 Česká republika), Sunhwa CHOI (410 Korejská republika), Sang Mi CHOI (410 Korejská republika), Přemysl SOUČEK (203 Česká republika), Jakub HORÁK (203 Česká republika), Blanka PEKÁROVÁ (203 Česká republika), Klaus PALME (276 Německo), Břetislav BRZOBOHATÝ (203 Česká republika) a Ildoo HWANG (410 Korejská republika)
Vydání
Plant Cell, United States, AMER SOC PLANT BIOLOGISTS, 2009, 1040-4651
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 9.293
Kód RIV
RIV/00216224:14310/09:00036886
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000270415700011
Klíčová slova česky
Histidine kinases CKI1
Klíčová slova anglicky
Histidine kinases CKI1
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 9. 4. 2010 16:05, doc. RNDr. Jan Hejátko, Ph.D.
V originále
The development and activity of the procambium and cambium, which ensure vascular tissue formation, is critical for overall plant architecture and growth. However, little is known about the molecular factors affecting the activity of vascular meristems and vascular tissue formation. Here we show that the sensor histidine kinase CKI1 and the cytokinin receptors AHK2 and AHK3 are important regulators of vascular tissue development in Arabidopsis shoots. Genetic modifications of CKI1 activity in Arabidopsis causes dysfunction of the two-component signaling pathway and defects in procambial cell maintenance and/or identity. CKI1 overexpression in protoplasts leads to cytokinin-independent activation of the two-component phosphorelay, and intracellular domains are responsible for cytokinin-independent activity of CKI1. CKI1 expression is restricted to vascular tissues in inflorescence stems, and CKI1 forms homodimers both in vitro and in planta. Loss-of-function ahk2 and ahk3 mutants and plants with reduced levels of endogenous cytokinins show defects in procambium proliferation and an absence of secondary growth. These results indicate that the cytokinin-independent activity of CKI1 and cytokinin-induced AHK2 and AHK3 are important for vascular bundle formation and biomass production in Arabidopsis.
Česky
The development and activity of the procambium and cambium, which ensure vascular tissue formation, is critical for overall plant architecture and growth. However, little is known about the molecular factors affecting the activity of vascular meristems and vascular tissue formation. Here we show that the sensor histidine kinase CKI1 and the cytokinin receptors AHK2 and AHK3 are important regulators of vascular tissue development in Arabidopsis shoots. Genetic modifications of CKI1 activity in Arabidopsis causes dysfunction of the two-component signaling pathway and defects in procambial cell maintenance and/or identity. CKI1 overexpression in protoplasts leads to cytokinin-independent activation of the two-component phosphorelay, and intracellular domains are responsible for cytokinin-independent activity of CKI1. CKI1 expression is restricted to vascular tissues in inflorescence stems, and CKI1 forms homodimers both in vitro and in planta. Loss-of-function ahk2 and ahk3 mutants and plants with reduced levels of endogenous cytokinins show defects in procambium proliferation and an absence of secondary growth. These results indicate that the cytokinin-independent activity of CKI1 and cytokinin-induced AHK2 and AHK3 are important for vascular bundle formation and biomass production in Arabidopsis.
Návaznosti
| LC06034, projekt VaV |
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| MSM0021622415, záměr |
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