Intracoronary delivery of bone marrow cells to the acutely
infarcted myocardium

J 2009

Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium

MELUZÍN, Jaroslav; Michal VLAŠÍN; Ladislav GROCH; Jiří MAYER; Leoš KŘEN et al.

Základní údaje

Originální název

Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium

Název česky

Intrakoronární podání buněk kostní dřeně u akutního infarktu myokardu

Autoři

Vydání

Cardiology, 2009, 0008-6312

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.637

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/09:00067142

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1159/000141462

UT WoS

000260892300003

Klíčová slova anglicky

Myocardial infarction; Bone marrow cells; Delivery technique

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 29. 4. 2014 10:06, Ing. Mgr. Věra Pospíšilíková

Anotace

ORIG CZ

V originále

Objectives: Intracoronary cell transplantation during catheter balloon inflations may be associated with adverse events. We studied the effectiveness of an alternative transplantation technique-intracoronary cell infusion. Methods: Fourteen pigs, which had survived acute myocardial infarction, were randomized into 2 treatment groups and 2 controls. Three days after infarction, 12 pigs underwent allogeneic intracoronary mononuclear bone marrow cell transplantation using either the standard technique (short-term cell injections during repeat balloon inflations, technique A, n = 6) or continuous intracoronary cell infusion without balloon inflations (technique B, n = 6). Implanted cells were stained with fluorescent dye. After transplantation, the pigs were euthanized and myocardial samples were analyzed by fluorescent microscopy. Results: The mean numbers of fluorescently labeled bone marrow cells in the infarction border zone, in the infarction mid-area and in the center of myocardial infarction were 84, 72 and 55 using technique A, and 29, 57 and 46 using technique B, respectively. The mean cell retention in the infarction border zone of 84 cells for technique A and 29 cells for technique B differed significantly (p = 0.034, two-tailed t test). Conclusion: The continuous intracoronary cell infusion technique is a less efficient cell delivery technique as compared with the standard technique using repeat intracoronary balloon inflations.

Česky

Continuální intrakoronární podání buněk kostní dřeně je méně účinné než použití standardní techniky využívající opakováné inflace katetrizačního balonku.

Návaznosti

MSM0021622402, záměr

Název: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časná diagnostika a léčba kardiovaskulárních chorob

MSM0021622430, záměr

Název: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Citovat

MELUZÍN, Jaroslav; Michal VLAŠÍN; Ladislav GROCH; Jiří MAYER; Leoš KŘEN; Petr RAUŠER; Boris TICHÝ; Ivan HORŇÁČEK; Martin KLABUSAY; Zdeněk KOŘÍSTEK; Michael DOUBEK; Šárka POSPÍŠILOVÁ; Ladislav DUŠEK; Jan SITAR; Stanislav PALŠA a Leona LEXMAULOVÁ. Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium. Cardiology. 2009, roč. 112, č. 2, s. 98-106. ISSN 0008-6312. Dostupné z: https://doi.org/10.1159/000141462.

@article{851199,
   author = {Meluzín, Jaroslav and Vlašín, Michal and Groch, Ladislav and Mayer, Jiří and Křen, Leoš and Raušer, Petr and Tichý, Boris and Horňáček, Ivan and Klabusay, Martin and Kořístek, Zdeněk and Doubek, Michael and Pospíšilová, Šárka and Dušek, Ladislav and Sitar, Jan and Palša, Stanislav and Lexmaulová, Leona},
   article_number = {2},
   doi = {https://doi.org/10.1159/000141462},
   keywords = {Myocardial infarction; Bone marrow cells; Delivery technique},
   language = {eng},
   issn = {0008-6312},
   journal = {Cardiology},
   title = {Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium},
   volume = {112},
   year = {2009}
}

TY  - JOUR
ID  - 851199
AU  - Meluzín, Jaroslav - Vlašín, Michal - Groch, Ladislav - Mayer, Jiří - Křen, Leoš - Raušer, Petr - Tichý, Boris - Horňáček, Ivan - Klabusay, Martin - Kořístek, Zdeněk - Doubek, Michael - Pospíšilová, Šárka - Dušek, Ladislav - Sitar, Jan - Palša, Stanislav - Lexmaulová, Leona
PY  - 2009
TI  - Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium
JF  - Cardiology
VL  - 112
IS  - 2
SP  - 98-106
EP  - 98-106
SN  - 00086312
KW  - Myocardial infarction
KW  - Bone marrow cells
KW  - Delivery technique
N2  - Objectives: Intracoronary cell transplantation during catheter balloon inflations may be associated with adverse events. We studied the effectiveness of an alternative transplantation technique-intracoronary cell infusion. Methods: Fourteen pigs, which had survived acute myocardial infarction, were randomized into 2 treatment groups and 2 controls. Three days after infarction, 12 pigs underwent allogeneic intracoronary mononuclear bone marrow cell transplantation using either the standard technique (short-term cell injections during repeat balloon inflations, technique A, n = 6) or continuous intracoronary cell infusion without balloon inflations (technique B, n = 6). Implanted cells were stained with fluorescent dye. After transplantation, the pigs were euthanized and myocardial samples were analyzed by fluorescent microscopy. Results: The mean numbers of fluorescently labeled bone marrow cells in the infarction border zone, in the infarction mid-area and in the center of myocardial infarction were 84, 72 and 55 using technique A, and 29, 57 and 46 using technique B, respectively. The mean cell retention in the infarction border zone of 84 cells for technique A and 29 cells for technique B differed significantly (p = 0.034, two-tailed t test). Conclusion: The continuous intracoronary cell infusion technique is a less efficient cell delivery technique as compared with the standard technique using repeat intracoronary balloon inflations.
ER  -

MELUZÍN, Jaroslav; Michal VLAŠÍN; Ladislav GROCH; Jiří MAYER; Leoš KŘEN; Petr RAUŠER; Boris TICHÝ; Ivan HORŇÁČEK; Martin KLABUSAY; Zdeněk KOŘÍSTEK; Michael DOUBEK; Šárka POSPÍŠILOVÁ; Ladislav DUŠEK; Jan SITAR; Stanislav PALŠA a Leona LEXMAULOVÁ. Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium. \textit{Cardiology}. 2009, roč.~112, č.~2, s.~98-106. ISSN~0008-6312. Dostupné z: https://doi.org/10.1159/000141462.

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