2009
Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array
DUBOVÝ, Petr; Marek DAVID; Ivana HRADILOVÁ SVÍŽENSKÁ a Ilona KLUSÁKOVÁZákladní údaje
Originální název
Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array
Název česky
Bilaterální kvantitativní změny lamininu 1 v dorzálních a ventrálních kořenech po unilaterální chronické konstrikci sciatického nervu - Imunofluorescenční analýza tkáně
Autoři
DUBOVÝ, Petr; Marek DAVID; Ivana HRADILOVÁ SVÍŽENSKÁ a Ilona KLUSÁKOVÁ
Vydání
9th European Meeting on Glial Cells in Health and Disease, 2009
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Francie
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 4.932
Označené pro přenos do RIV
Ne
Organizační jednotka
Lékařská fakulta
ISSN
UT WoS
Klíčová slova česky
behaviorální testy; bilaterální reakce kořenů; dorzální kořen; endoneurium; kořeny spinálního nervu; laminin 1; neuropatická bolest; periferní nervová soustava; unilaterální konstrikce sciatického nervu; ventrální kořen
Klíčová slova anglicky
behavioral tests; bilateral roots reaction; dorsal root; endoneurium; laminin-1; neuropathic pain; peripheral nervous system; spinal nerve roots; unilateral constriction of sciatic nerve; ventral root
Štítky
Příznaky
Mezinárodní význam
Změněno: 6. 4. 2010 12:04, Mgr. Marek David, Ph.D.
V originále
Bilateral changes of immunofluorescence for laminin-1 (lam-1) were investigated in the rat spinal nerve roots (L4-L5) following neuropathic pain induction by unilateral sciatic nerve ligature. Induction of neuropathic pain was confirmed in experimental animals by behavioral tests of mechanical allodynia and thermal hyperalgesia. Dorsal and ventral roots of naive rats displayed lam-1 immunostaining in the basal laminae on outer surface of Schwann cell/axon units, perineurial sheaths and blood vessels. Significantly higher brightness of immunofluorescence for lam-1 was observed in the ventral than dorsal roots removed from naive rats as well as those operated on sciatic nerve ligature and surviving for 1 or 3 days. Chronic constriction injury of sciatic nerve for 7 and 14 days results in reduction of different levels of immunofluorescence brightness between dorsal and ventral roots. In comparison to naive and sham-operated animals, apparent bilateral decrease of immunofluorescence brightness for lam-1 was found in both dorsal and ventral roots 7 days after unilateral nerve injury. Although the pattern of lam-1 alterations in both ipsi- and contralateral roots was similar, changes of immunofluorescence were more evident in ipsilateral than contralateral roots. The results indicate that quantitative changes of immunofluorescence for lam-1 are present bilaterally in proximal segments of peripheral nerve far from unilateral chronic constriction injury inducing neuropathic pain. This suggests a possible mechanism for misbalance alongside the afferent and motor axons, the involvement of which in neuropathic pain induction is assumed.
Česky
Bilateral changes of immunofluorescence for laminin-1 (lam-1) were investigated in the rat spinal nerve roots (L4-L5) following neuropathic pain induction by unilateral sciatic nerve ligature. Induction of neuropathic pain was confirmed in experimental animals by behavioral tests of mechanical allodynia and thermal hyperalgesia. Dorsal and ventral roots of naive rats displayed lam-1 immunostaining in the basal laminae on outer surface of Schwann cell/axon units, perineurial sheaths and blood vessels. Significantly higher brightness of immunofluorescence for lam-1 was observed in the ventral than dorsal roots removed from naive rats as well as those operated on sciatic nerve ligature and surviving for 1 or 3 days. Chronic constriction injury of sciatic nerve for 7 and 14 days results in reduction of different levels of immunofluorescence brightness between dorsal and ventral roots. In comparison to naive and sham-operated animals, apparent bilateral decrease of immunofluorescence brightness for lam-1 was found in both dorsal and ventral roots 7 days after unilateral nerve injury. Although the pattern of lam-1 alterations in both ipsi- and contralateral roots was similar, changes of immunofluorescence were more evident in ipsilateral than contralateral roots. The results indicate that quantitative changes of immunofluorescence for lam-1 are present bilaterally in proximal segments of peripheral nerve far from unilateral chronic constriction injury inducing neuropathic pain. This suggests a possible mechanism for misbalance alongside the afferent and motor axons, the involvement of which in neuropathic pain induction is assumed.
Návaznosti
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