Combined Contactless Conductometric, Photometric and
Fluorimetric Single Point Detector for Capillary Separation
Methods

J 2010

Combined Contactless Conductometric, Photometric and Fluorimetric Single Point Detector for Capillary Separation Methods

RYVOLOVÁ, Markéta, Jan PREISLER, František FORET, Peter HAUSER, Pavel KRÁSENSKÝ et. al.

Základní údaje

Originální název

Combined Contactless Conductometric, Photometric and Fluorimetric Single Point Detector for Capillary Separation Methods

Název česky

Kombinovaný bezkontaktní vodivostní, fotometrický a fluorimetrický jednobodový detektor pro kapilární separační metody

Autoři

RYVOLOVÁ, Markéta (203 Česká republika, domácí), Jan PREISLER (203 Česká republika, garant, domácí), František FORET (203 Česká republika), Peter HAUSER (756 Švýcarsko), Pavel KRÁSENSKÝ (203 Česká republika, domácí), Brett PAULL (372 Irsko) a Mirek MACKA (372 Irsko)

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2010, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.874

Kód RIV

RIV/00216224:14310/10:00042881

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000273265700027

Klíčová slova anglicky

detection; capillary separation methods; combined detector; photometric detection; fluorimetric detection; C4D

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 7. 2013 15:39, Ing. Andrea Mikešková

Anotace

V originále

This work for the first time combines three on-capillary detection methods, namely capacitively coupled contactless conductometric (C4D), photometric (PD) and fluorimetric (FD), in a single (identical) point of detection cell, allowing concurrent measurements at a single point of detection for use in capillary electrophoresis, capillary electrochromatography and capillary/nano-liquid chromatography. The novel design is based on a standard 6.3 mm i.d. fiber-optic SMA adapter with a drilled opening for the separation capillary to go through, to which two concentrically positioned C4D detection electrodes with a detection gap of 7 mm were added on its each side acting simultaneously as capillary guides. The optical fibers in the SMA adapter were used for the photometric signal (absorbance) and another optical fiber at a 45 degree angle to the capillary was applied to collect the emitted light for FD. Light emitting diodes (255 nm and 470 nm) were used as light sources for the PD and FD detection modes. LOD values were determined under flow-injection conditions to exclude any stacking effects: For the 470 nm LED LODs for FD and PD were for fluorescein (1x10-8 mol/L) and tartrazine (6x10-6 mol/L), respectively and LOD for the C4D was for magnesium chloride (5x10-7 mol/L). The advantage of the three different detection signals in a single point is demonstrated in capillary electrophoresis using model mixtures and samples including a mixture of fluorescent and non fluorescent dyes and common ions, underivatized amino acids, and a fluorescently labeled digest of bovine serum albumin.

Návaznosti

GA203/09/1025, projekt VaV

Název: Multidetekční platforma pro mikrokolonové separace proteinů a peptidů

Investor: Grantová agentura ČR, Multidetekční platforma pro mikrokolonové separace proteinů a peptidů

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

RYVOLOVÁ, Markéta, Jan PREISLER, František FORET, Peter HAUSER, Pavel KRÁSENSKÝ, Brett PAULL a Mirek MACKA. Combined Contactless Conductometric, Photometric and Fluorimetric Single Point Detector for Capillary Separation Methods. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2010, roč. 82, č. 1, s. 129-135. ISSN 0003-2700.

@article{855254,
   author = {Ryvolová, Markéta and Preisler, Jan and Foret, František and Hauser, Peter and Krásenský, Pavel and Paull, Brett and Macka, Mirek},
   article_location = {Washington, D.C., USA},
   article_number = {1},
   keywords = {detection; capillary separation methods; combined detector; photometric detection; fluorimetric detection; C4D},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Combined Contactless Conductometric, Photometric and Fluorimetric Single Point Detector for Capillary Separation Methods},
   url = {http://pubs.acs.org/journals/ancham/index.html},
   volume = {82},
   year = {2010}
}

TY  - JOUR
ID  - 855254
AU  - Ryvolová, Markéta - Preisler, Jan - Foret, František - Hauser, Peter - Krásenský, Pavel - Paull, Brett - Macka, Mirek
PY  - 2010
TI  - Combined Contactless Conductometric, Photometric and Fluorimetric Single Point Detector for Capillary Separation Methods
JF  - Analytical Chemistry
VL  - 82
IS  - 1
SP  - 129-135
EP  - 129-135
PB  - American Chemical Society
SN  - 00032700
KW  - detection
KW  - capillary separation methods
KW  - combined detector
KW  - photometric detection
KW  - fluorimetric detection
KW  - C4D
UR  - http://pubs.acs.org/journals/ancham/index.html
N2  - This work for the first time combines three on-capillary detection methods, namely capacitively coupled contactless conductometric (C4D), photometric (PD) and fluorimetric (FD), in a single (identical) point of detection cell, allowing concurrent measurements at a single point of detection for use in capillary electrophoresis, capillary electrochromatography and capillary/nano-liquid chromatography. The novel design is based on a standard 6.3 mm i.d. fiber-optic SMA adapter with a drilled opening for the separation capillary to go through, to which two concentrically positioned C4D detection electrodes with a detection gap of 7 mm were added on its each side acting simultaneously as capillary guides. The optical fibers in the SMA adapter were used for the photometric signal (absorbance) and another optical fiber at a 45 degree angle to the capillary was applied to collect the emitted light for FD. Light emitting diodes (255 nm and 470 nm) were used as light sources for the PD and FD detection modes. LOD values were determined under flow-injection conditions to exclude any stacking effects: For the 470 nm LED LODs for FD and PD were for fluorescein (1x10-8 mol/L) and tartrazine (6x10-6 mol/L), respectively and LOD for the C4D was for magnesium chloride (5x10-7 mol/L). The advantage of the three different detection signals in a single point is demonstrated in capillary electrophoresis using model mixtures and samples including a mixture of fluorescent and non fluorescent dyes and common ions, underivatized amino acids, and a fluorescently labeled digest of bovine serum albumin.
ER  -

RYVOLOVÁ, Markéta, Jan PREISLER, František FORET, Peter HAUSER, Pavel KRÁSENSKÝ, Brett PAULL a Mirek MACKA. Combined Contactless Conductometric, Photometric and Fluorimetric Single Point Detector for Capillary Separation Methods. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2010, roč.~82, č.~1, s.~129-135. ISSN~0003-2700.

Podrobný výpis o publikaci