D 2009

PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS

LIŠKOVÁ, Marcela; Karel KLEPÁRNÍK; Věra HEZINOVÁ; Ilona SVOBODOVÁ; Jan PŘIKRYL et al.

Základní údaje

Originální název

PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS

Název česky

PREPARATION AND MODIFICATION OF QUANTUM DOTS AS SELECTIVE PROBES IN BIOANALYSIS

Autoři

LIŠKOVÁ, Marcela; Karel KLEPÁRNÍK; Věra HEZINOVÁ; Ilona SVOBODOVÁ; Jan PŘIKRYL; František FORET a Pavel PAZDERA

Vydání

Bratislava, ChemZi 5/9 2009, 61. Zjazd chemikov, Vysoké Tatry, Slovensko, 2 s. 2009

Nakladatel

Slovenská chemická spoločnost

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/09:00037756

Organizační jednotka

Přírodovědecká fakulta

ISSN

Klíčová slova česky

Quantum dots ;photoluminescent probes;immunolabeling

Klíčová slova anglicky

Quantum dots ;photoluminescent probes;immunolabeling

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 5. 10. 2010 07:17, doc. RNDr. Pavel Pazdera, CSc.

Anotace

ORIG CZ

V originále

Quantum dots (QD) show a great potential as photoluminescent probes at cellular level, including in vivo immunolabeling or cellular tracking. The main advantages of quantum dots, when compared with the conventional organic fluorescent dyes, are practically no photobleaching, wide excitation and narrow emission spectra and size dependent emission wavelength. Typical materials for the preparation of quantum dots are elements of II - VI or III - V group. We have prepared CdTe water soluble quantum dots of sizes 2 - 4,5 nm by the chemical reaction between CdCl2 and NaHTe at the presence of 3-mercaptopropionic acid. Thus, the photoluminescence emission maxima reach the values 520 - 700 nm. The emission spectra are narrow with the bandwidth of about 58 nm at the half height of its intensity, while the excitation band lies in the range from 300 to 550 nm. There is a lot of simple conjugation techniques for linking of QD with biomolecules to use them as selective probes in immunolabeling.

Česky

Quantum dots (QD) show a great potential as photoluminescent probes at cellular level, including in vivo immunolabeling or cellular tracking. The main advantages of quantum dots, when compared with the conventional organic fluorescent dyes, are practically no photobleaching, wide excitation and narrow emission spectra and size dependent emission wavelength. Typical materials for the preparation of quantum dots are elements of II - VI or III - V group. We have prepared CdTe water soluble quantum dots of sizes 2 - 4,5 nm by the chemical reaction between CdCl2 and NaHTe at the presence of 3-mercaptopropionic acid. Thus, the photoluminescence emission maxima reach the values 520 - 700 nm. The emission spectra are narrow with the bandwidth of about 58 nm at the half height of its intensity, while the excitation band lies in the range from 300 to 550 nm. There is a lot of simple conjugation techniques for linking of QD with biomolecules to use them as selective probes in immunolabeling.