2009
Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
BOUCHAL, Pavel; Iva STRUHÁROVÁ; Eva BUDINSKÁ; Ondrej ŠEDO; Tereza VYHLÍDALOVÁ et al.Základní údaje
Originální název
Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
Název česky
Odhalování regulačního okruhu regulátorů FNR-typu u bakterie Paracoccus denitrificans pomocí proteomických, transkriptomických a bioinformatických nástrojů
Název anglicky
Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches
Autoři
BOUCHAL, Pavel; Iva STRUHÁROVÁ; Eva BUDINSKÁ; Ondrej ŠEDO; Tereza VYHLÍDALOVÁ; Zbyněk ZDRÁHAL; Rob VAN SPANNING a Igor KUČERA
Vydání
2009 SPS Scientific Meeting: "Proteome Dynamics: Protein Quantification in Time and Space" 2009
Další údaje
Jazyk
čeština
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14310/09:00029725
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
proteome Paracoccus denitrificans
Příznaky
Mezinárodní význam
Změněno: 29. 4. 2011 12:00, doc. Mgr. Pavel Bouchal, Ph.D.
V originále
The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three Fnr-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this study, we aimed to discover the role of Fnr-family transcription regulators in the aerobic-semiaerobic switch at global level. Methods and Materials: We analyzed the protein composition of four Paracoccus denitrificans strains (wild type, FnrP-, NNRand NarR- mutant strains) grown aerobically, semiaerobically and semiearobically in the presence of nitrate using two-dimensional gel electropohoresis with MS/MS protein identification, with data validation at the transcript (qRT-PCR) and genome (FNR-binding sequences) levels. Results: Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins gene products were identified using mass spectrometry.
Anglicky
The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three Fnr-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this study, we aimed to discover the role of Fnr-family transcription regulators in the aerobic-semiaerobic switch at global level. Methods and Materials: We analyzed the protein composition of four Paracoccus denitrificans strains (wild type, FnrP-, NNRand NarR- mutant strains) grown aerobically, semiaerobically and semiearobically in the presence of nitrate using two-dimensional gel electropohoresis with MS/MS protein identification, with data validation at the transcript (qRT-PCR) and genome (FNR-binding sequences) levels. Results: Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins gene products were identified using mass spectrometry.
Návaznosti
| GP203/07/P471, projekt VaV |
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| MSM0021622413, záměr |
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