2010
microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients
HEŽOVÁ, Renata; Ondřej SLABÝ; Petra FALTEJSKOVÁ; Zuzana MIKULKOVÁ; Ivana BUREŠOVÁ et. al.Základní údaje
Originální název
microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients
Název česky
microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients
Autoři
HEŽOVÁ, Renata (203 Česká republika); Ondřej SLABÝ (203 Česká republika, garant); Petra FALTEJSKOVÁ (203 Česká republika); Zuzana MIKULKOVÁ (203 Česká republika); Ivana BUREŠOVÁ (203 Česká republika); Karthick Raja MUTHU RAJA (356 Indie); Jan HODEK (203 Česká republika); Jaroslava OVESNÁ (203 Česká republika) a Jaroslav MICHÁLEK (203 Česká republika)
Vydání
Cellular Immunology, Elsevier, 2010, 0008-8749
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30200 3.2 Clinical medicine
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.575
Kód RIV
RIV/00216224:14110/10:00040729
Organizační jednotka
Lékařská fakulta
UT WoS
000273594200003
Klíčová slova anglicky
microRNA; Expression profile; Regulatory T cells; Diabetes mellitus type 1
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 4. 8. 2010 13:19, prof. RNDr. Ondřej Slabý, Ph.D.
V originále
We performed miRNAs profiling in a population of regulatory T celss (Tregs) separated from peripheral blood of five type 1 diabetic patients and six healthy donors. For more detailed molecular char- acterisation of Tregs, we additionally compared miRNAs expression profiles of Tregs and conventional T cells. Tregs were isolated according to CD3+, CD4+, CD25hi+ and CD127low by flow cytometry, and miRNA expression profiling was performed using TaqMan Array Human MicroRNA Panel-1 (384-well low density array). In Tregs of diabetic patients we found significantly increased expression of miRNA-510 (p = 0.05) and decreased expression of both miRNA-342 (p< 0.0001) and miRNA-191 (p = 0.0079). When comparing Tregs and T cells, we revealed that Tregs had significant higher expression of miRNA-146a and lower expression of eight specific miRNAs (20b, 31, 99a, 100, 125b, 151, 335, and 365). This is the first study demonstrating changes in miRNA expression profiles occurring in Tregs of T1D patients and a miRNAs signature of adult Tregs.
Česky
We performed miRNAs profiling in a population of regulatory T celss (Tregs) separated from peripheral blood of five type 1 diabetic patients and six healthy donors. For more detailed molecular char- acterisation of Tregs, we additionally compared miRNAs expression profiles of Tregs and conventional T cells. Tregs were isolated according to CD3+, CD4+, CD25hi+ and CD127low by flow cytometry, and miRNA expression profiling was performed using TaqMan Array Human MicroRNA Panel-1 (384-well low density array). In Tregs of diabetic patients we found significantly increased expression of miRNA-510 (p = 0.05) and decreased expression of both miRNA-342 (p< 0.0001) and miRNA-191 (p = 0.0079). When comparing Tregs and T cells, we revealed that Tregs had significant higher expression of miRNA-146a and lower expression of eight specific miRNAs (20b, 31, 99a, 100, 125b, 151, 335, and 365). This is the first study demonstrating changes in miRNA expression profiles occurring in Tregs of T1D patients and a miRNAs signature of adult Tregs.
Návaznosti
| NR9355, projekt VaV |
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| 2B08066, projekt VaV |
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