Metallomics of melanoma animal tissues

J 2009

Metallomics of melanoma animal tissues

MASAŘÍK, Michal, Ondřej ZÍTKA, Dalibor HÚSKA, Soňa KŘÍŽKOVÁ, Ján STRNADEL et. al.

Základní údaje

Originální název

Metallomics of melanoma animal tissues

Název česky

Metalomika ve vztahu k melanomu živočišných tkání

Autoři

MASAŘÍK, Michal (203 Česká republika, garant), Ondřej ZÍTKA (203 Česká republika), Dalibor HÚSKA (203 Česká republika), Soňa KŘÍŽKOVÁ (203 Česká republika), Ján STRNADEL (203 Česká republika), Vratislav HORÁK (203 Česká republika), Tomáš VACULOVIČ (203 Česká republika), Karel NOVOTNÝ (203 Česká republika), Viktor KANICKÝ (203 Česká republika), Josef KAISER (203 Česká republika), Vojtěch ADAM (203 Česká republika) a René KIZEK (203 Česká republika)

Vydání

International Journal of Molecular Medicine, 2009, 1107-3756

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.980

Kód RIV

RIV/00216224:14110/09:00028764

Organizační jednotka

Lékařská fakulta

UT WoS

000270497500179

Klíčová slova anglicky

metallomics; LIBS; electrochemistry; melanoma

Změněno: 9. 4. 2010 10:33, doc. Mgr. Karel Novotný, Ph.D.

Anotace

ORIG CZ

V originále

Metallomics and metalloproteomics are emerging fields addressing the role, uptake, transport and storage of trace metals essential for life. There are several main approaches that are being developed in metallomics and metalloproteomics including both detection of levels and spatial distribution of heavy metals and determination of heavy-metal-contained proteins. The aim of this work is detection of copper and zinc in healthy and tumour tissues of miniature pigs by using of the laser induced breakdown spectroscopy (LIBS). Concentration of heavy metal transporting protein metallothionein (MT) was determined by Brdicka reaction. Tissue cryosections were obtained from the MeLiM strain of miniature pigs with hereditary melanoma, particularly from healthy skin, cutaneous nodular melanomas and metastases in the liver, spleen and lymph nodes. Using LIBS we measured maps of spatial distribution of the essential heavy metals in cryosections and found that the maps of healthy and tumour cryosection markedly differed. The highest content of MT was determined in the tumours localised on the back of animals and was nearly 500 ug of MT per gram of tissue.

Česky

Cílem této práce byla analýza metaloproteinů pomocí elektrochemie a laserem indukované spektroskopie.

Návaznosti

IAA401990701, projekt VaV

Název: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu

Citovat

MASAŘÍK, Michal, Ondřej ZÍTKA, Dalibor HÚSKA, Soňa KŘÍŽKOVÁ, Ján STRNADEL, Vratislav HORÁK, Tomáš VACULOVIČ, Karel NOVOTNÝ, Viktor KANICKÝ, Josef KAISER, Vojtěch ADAM a René KIZEK. Metallomics of melanoma animal tissues. International Journal of Molecular Medicine. 2009, roč. 24, Suppl. 1, s. S49, 2 s. ISSN 1107-3756.

@article{868024,
   author = {Masařík, Michal and Zítka, Ondřej and Húska, Dalibor and Křížková, Soňa and Strnadel, Ján and Horák, Vratislav and Vaculovič, Tomáš and Novotný, Karel and Kanický, Viktor and Kaiser, Josef and Adam, Vojtěch and Kizek, René},
   article_number = {Suppl. 1},
   keywords = {metallomics; LIBS; electrochemistry; melanoma},
   language = {eng},
   issn = {1107-3756},
   journal = {International Journal of Molecular Medicine},
   title = {Metallomics of melanoma animal tissues},
   volume = {24},
   year = {2009}
}

TY  - JOUR
ID  - 868024
AU  - Masařík, Michal - Zítka, Ondřej - Húska, Dalibor - Křížková, Soňa - Strnadel, Ján - Horák, Vratislav - Vaculovič, Tomáš - Novotný, Karel - Kanický, Viktor - Kaiser, Josef - Adam, Vojtěch - Kizek, René
PY  - 2009
TI  - Metallomics of melanoma animal tissues
JF  - International Journal of Molecular Medicine
VL  - 24
IS  - Suppl. 1
SP  - S49
EP  - S49
SN  - 11073756
KW  - metallomics
KW  - LIBS
KW  - electrochemistry
KW  - melanoma
N2  - Metallomics and metalloproteomics are emerging fields addressing the role, uptake, transport and storage of trace metals essential for life. There are several main approaches that are being developed in metallomics and metalloproteomics including both detection of levels and spatial distribution of heavy metals and determination of heavy-metal-contained proteins. The aim of this work is detection of copper and zinc in healthy and tumour tissues of miniature pigs by using of the laser induced breakdown spectroscopy (LIBS). Concentration of heavy metal transporting protein metallothionein (MT) was determined by Brdicka reaction. Tissue cryosections were obtained from the MeLiM strain of miniature pigs with hereditary melanoma, particularly from healthy skin, cutaneous nodular melanomas and metastases in the liver, spleen and lymph nodes. Using LIBS we measured maps of spatial distribution of the essential heavy metals in cryosections and found that the maps of healthy and tumour cryosection markedly differed. The highest content of MT was determined in the tumours localised on the back of animals and was nearly 500 ug of MT per gram of tissue.
ER  -

MASAŘÍK, Michal, Ondřej ZÍTKA, Dalibor HÚSKA, Soňa KŘÍŽKOVÁ, Ján STRNADEL, Vratislav HORÁK, Tomáš VACULOVIČ, Karel NOVOTNÝ, Viktor KANICKÝ, Josef KAISER, Vojtěch ADAM a René KIZEK. Metallomics of melanoma animal tissues. \textit{International Journal of Molecular Medicine}. 2009, roč.~24, Suppl. 1, s.~S49, 2 s. ISSN~1107-3756.

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