Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

BÜYÜKGÜZEL, Ender, Pavel HYRŠL a Kemal BÜYÜKGÜZEL. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology. 2010, roč. 156, č. 2, s. 176-183, 7 s. ISSN 1095-6433.

Základní údaje

• Originální název: Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.
• Autoři: BÜYÜKGÜZEL, Ender (792 Turecko), Pavel HYRŠL (203 Česká republika, garant) a Kemal BÜYÜKGÜZEL (792 Turecko).
• Vydání: Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology, 2010, 1095-6433.

Další údaje

• Originální jazyk: angličtina
• Typ výsledku: Článek v odborném periodiku
• Obor: 30105 Physiology
• Stát vydavatele: Spojené státy
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 2.134
• Kód RIV: RIV/00216224:14310/10:00043258
• Organizační jednotka: Přírodovědecká fakulta
• UT WoS: 000277748500003
• Klíčová slova anglicky: Eicosanoids; Galleria mellonella; antioxidant enzymes; oxidative stress; pro-oxidant
• Příznaky: Mezinárodní význam

Anotace

We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin, the phospholipase A2 inhibitor,dexamethasone and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing xanthotoxin (XA) for 2 days. Treating seventh-instar larvae of G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. Relative to control, eicosanoid biosynthesis inhibitors also resulted in impaired MDA content and antioxidant enzyme activities. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.

Anotace česky

We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin, the phospholipase A2 inhibitor,dexamethasone and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing xanthotoxin (XA) for 2 days. Treating seventh-instar larvae of G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. Relative to control, eicosanoid biosynthesis inhibitors also resulted in impaired MDA content and antioxidant enzyme activities. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.