Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.

BÜYÜKGÜZEL, Ender, Pavel HYRŠL and Kemal BÜYÜKGÜZEL. Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology. 2010, vol. 156, No 2, p. 176-183, 7 pp. ISSN 1095-6433.

Basic information

• Original name: Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L.
• Authors: BÜYÜKGÜZEL, Ender (792 Turkey), Pavel HYRŠL (203 Czech Republic, guarantor) and Kemal BÜYÜKGÜZEL (792 Turkey).
• Edition: Comparative Biochemistry and Physiology A - Molecular & Integrative Physiology, 2010, 1095-6433.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30105 Physiology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.134
• RIV identification code: RIV/00216224:14310/10:00043258
• Organization unit: Faculty of Science
• UT WoS: 000277748500003
• Keywords in English: Eicosanoids; Galleria mellonella; antioxidant enzymes; oxidative stress; pro-oxidant
• Tags: International impact

Abstract

We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin, the phospholipase A2 inhibitor,dexamethasone and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing xanthotoxin (XA) for 2 days. Treating seventh-instar larvae of G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. Relative to control, eicosanoid biosynthesis inhibitors also resulted in impaired MDA content and antioxidant enzyme activities. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.

Abstract (in Czech)

We posed the hypothesis that eicosanoids also mediate antioxidant enzymatic defense reactions to pro-oxidant challenge. To test this idea, we reared first-instar larvae of Galleria mellonella (L.) with the lypoxygenase inhibitor, esculetin, the phospholipase A2 inhibitor,dexamethasone and the dual inhibitor of cyclooxygenase and lipoxygenase, phenidone to seventh-instars. Newly ecdysed seventh-instars were then fed on artificial diet containing xanthotoxin (XA) for 2 days. Treating seventh-instar larvae of G. mellonella with XA induced lipid peroxidation and protein carbonylation as evident from the increased content of malondialdehyde (MDA) and protein carbonyls respectively, and antioxidative enzymatic response in a dose-dependent manner. Relative to control, eicosanoid biosynthesis inhibitors also resulted in impaired MDA content and antioxidant enzyme activities. We infer from these findings that eicosanoids mediate insect antioxidant enzymatic responses to dietary pro-oxidants.