MAŠOVÁ, Šárka, Petr WANDROL and Vlastimil BARUŠ. COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE. In Mikroskopie 2010. 2010.
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Basic information
Original name COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
Name in Czech Srovnání ESEM a SEM v morfologické studii paraztické hlístice
Authors MAŠOVÁ, Šárka (203 Czech Republic, guarantor, belonging to the institution), Petr WANDROL (203 Czech Republic) and Vlastimil BARUŠ (203 Czech Republic, belonging to the institution).
Edition Mikroskopie 2010, 2010.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/10:00043372
Organization unit Faculty of Science
Keywords (in Czech) Nematoda; ESEM; SEM; hlístice; Heterotis niloticus; Brevimulticaecum heterotis
Keywords in English Nematoda; ESEM; SEM; nematode; Heterotis niloticus; Brevimulticaecum heterotis
Tags Africa, Brevimulticaecum, morphology, Nematoda, nematode, SEM study, West Africa
Changed by Changed by: RNDr. Šárka Mašová, Ph.D., učo 106026. Changed: 24/3/2011 17:24.
Abstract
Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribe its external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs and coated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies.
Links
GD526/09/H025, research and development projectName: Evolučně-ekologická analýza společenstev a populací
Investor: Czech Science Foundation, Evolutionary ecological analysis of communities and populations
LC522, research and development projectName: ICHTYOPARAZITOLOGIE - centrum základního výzkumu
Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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