COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE

MAŠOVÁ, Šárka, Petr WANDROL and Vlastimil BARUŠ. COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE. In Mikroskopie 2010. 2010.

Basic information

• Original name: COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE
• Name in Czech: Srovnání ESEM a SEM v morfologické studii paraztické hlístice
• Authors: MAŠOVÁ, Šárka (203 Czech Republic, guarantor, belonging to the institution), Petr WANDROL (203 Czech Republic) and Vlastimil BARUŠ (203 Czech Republic, belonging to the institution).
• Edition: Mikroskopie 2010, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00043372
• Organization unit: Faculty of Science
• Keywords (in Czech): Nematoda; ESEM; SEM; hlístice; Heterotis niloticus; Brevimulticaecum heterotis
• Keywords in English: Nematoda; ESEM; SEM; nematode; Heterotis niloticus; Brevimulticaecum heterotis
• Tags: Africa, Brevimulticaecum, morphology, Nematoda, nematode, SEM study, West Africa

Abstract

Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribe its external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs and coated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies.

Links

• GD526/09/H025, research and development project: Name: Evolučně-ekologická analýza společenstev a populací

Investor: Czech Science Foundation, Evolutionary ecological analysis of communities and populations

• LC522, research and development project: Name: ICHTYOPARAZITOLOGIE - centrum základního výzkumu

Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time