2009
Whole genome amplification and analysis: genome differences in the genus Treponema.
MIKALOVÁ, Lenka; Michal STROUHAL; Petra MATĚJKOVÁ POSPÍŠILOVÁ; David ŠMAJS; George M. WEINSTOCK et. al.Základní údaje
Originální název
Whole genome amplification and analysis: genome differences in the genus Treponema.
Autoři
Vydání
3rd FEMS Congress of European Microbiologists. Abstract CD, 1749. 2009
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
Genetika a molekulární biologie
Stát vydavatele
Švédsko
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Lékařská fakulta
Klíčová slova česky
Trepomema pallidum; WGF; tpr geny
Klíčová slova anglicky
Trepomema pallidum; whole genome fingerprinting; tpr genes
Změněno: 25. 2. 2010 14:25, Mgr. Marie Zobaníková, Ph.D.
Anotace
V originále
Genome comparison of 6 treponemal strains including Mexico A, DAL-1 (T. pallidum subsp. pallidum), Gauthier, CDC-2 (T. pallidum subsp. pertenue), Bosnia A (T. pallidum subsp. endemicum) and Fribourg-Blanc (unclassified simian isolate) with the sequenced genome of the syphilis spirochete TPA strain Nichols. The whole genome fingerprinting technique was used for identification of heterologous regions in the chromosomal DNA of analyzed strains. All analyzed strains contained two regions of repetitive sequences (TP0433-434, TP0470) with strain specific repeat numbers. In the yaws and simian strain genomes, three specific indels were observed. Strain specific changes comprised e. g. insertion of 58 bp (TP0136) in DAL-1, deletion of 48 bp (TP0548) and insertion of 0.5 kb (TP0695-697) in Fribourg-Blanc. Genomes of T. p. pallidum, T. p. pertenue, T. p. endemicum and unclassified simian isolate are closely related and most of the observed sequence differences are localized in tpr genes and in their vinicity.
Návaznosti
| GA310/07/0321, projekt VaV |
| ||
| MSM0021622415, záměr |
| ||
| NR8967, projekt VaV |
|