Characterization of uropathogenic E. coli strains with reduced
susceptibility or resistance to extended spectrum
cephalosporines.

a 2009

Characterization of uropathogenic E. coli strains with reduced susceptibility or resistance to extended spectrum cephalosporines.

ČERNOHORSKÁ, Lenka, Zuzana VALIŠOVÁ, Vladana WOZNICOVÁ a David ŠMAJS

Základní údaje

Originální název

Characterization of uropathogenic E. coli strains with reduced susceptibility or resistance to extended spectrum cephalosporines.

Autoři

ČERNOHORSKÁ, Lenka, Zuzana VALIŠOVÁ, Vladana WOZNICOVÁ a David ŠMAJS

Vydání

Control and Mangement of Pathogenic Escherichia coli. 2009

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

rezistence k cefalosporinům, uropatogenní E. coli

Klíčová slova anglicky

resistance to cephalosporines; uropathogenic E. coli

Změněno: 1. 4. 2010 15:09, MUDr. Lenka Černohorská, Ph.D.

Anotace

ORIG CZ

V originále

AmpC beta-lactamases and ESBL are enzymes produced by a range of Gram-negative bacteria and able to hydrolyse penicillins, monobactams, cephalosporins and cephamycins and are now big epidemiological, diagnostic and therapeutical problem. During the period June 08- June 09 we have collected 106 E.coli isolates showing reduced susceptibility to extended-spectrum cephalosporines from the urine specimens of hospitalised patients with diagnosis of urinary tract infection. ESBL production was confirmed by DDST (double-disk synergy test). We also ascertained AmpC production. The production of inducible AmpC was assayed by modified DDST and that of constitutive AmpC was tested on agar containing oxacillin as an AmpC inhibitor. All producers were tested for antimicrobial susceptibility to amikacin, imipenem, meropenem, colisitin, piperacillin/tazobactam, cefepime, cefoperazone/sulbactam and tigecycline by the disk diffusion method. We also used modified Christensen method to find out the ability of isolates to produce biofilm. There were detected 88 (83.01 %) ESBL producers, 15 (14.15 %) constitutive AmpC producers, 2 (1.89 %) isolates producing both ESBL and constitutive AmpC and 1 (0.94 %) strain producing both ESBL and inducible AmpC. Eighty eight, i.e. 83.02 % of tested strains were susceptible to amikacin, 105 (99.06 %) to imipenem as well as to meropenem, 103 (97.17 %) to colistin, 43 ( 40.57 %) to piperacilllin/tazobactam, 82 (77.36 %) to cefepime and 49 (46.22 %) to cefoperazone/sulbactam. All the tested strains were susceptible to tigecycline. Biofilm was produced by 6 (5.66 %) strains.

Česky

Sledovali jsme produkci ESBL a AmpC u močových kmenů E. coli.

Návaznosti

NS9665, projekt VaV

Název: Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Investor: Ministerstvo zdravotnictví ČR, Molekulární typizace bakteriocinů produkovaných kmeny Escherichia coli z klinického materiálu: identifikace kolicinů a mikrocinů v souborech komensálních a patogenních kmenů

Citovat

ČERNOHORSKÁ, Lenka, Zuzana VALIŠOVÁ, Vladana WOZNICOVÁ a David ŠMAJS. Characterization of uropathogenic E. coli strains with reduced susceptibility or resistance to extended spectrum cephalosporines. In Control and Mangement of Pathogenic Escherichia coli. 2009.

@proceedings{873382,
   author = {Černohorská, Lenka and Vališová, Zuzana and Woznicová, Vladana and Šmajs, David},
   booktitle = {Control and Mangement of Pathogenic Escherichia coli.},
   keywords = {resistance to cephalosporines; uropathogenic E. coli},
   language = {eng},
   title = {Characterization of uropathogenic E. coli strains with reduced susceptibility or resistance to extended spectrum cephalosporines.},
   year = {2009}
}

TY  - CONF
ID  - 873382
AU  - Černohorská, Lenka - Vališová, Zuzana - Woznicová, Vladana - Šmajs, David
PY  - 2009
TI  - Characterization of uropathogenic E. coli strains with reduced susceptibility or resistance to extended spectrum cephalosporines.
KW  - resistance to cephalosporines
KW  - uropathogenic E. coli
N2  - AmpC beta-lactamases and ESBL are enzymes produced by a range of Gram-negative bacteria and able to hydrolyse penicillins, monobactams, cephalosporins and cephamycins and are now big epidemiological, diagnostic and therapeutical problem. During the period June 08- June 09 we have collected 106 E.coli isolates showing reduced susceptibility to extended-spectrum cephalosporines from the urine specimens of hospitalised patients with diagnosis of urinary tract infection. ESBL production was confirmed by DDST (double-disk synergy test). We also ascertained AmpC production. The production of inducible AmpC was assayed by modified DDST and that of constitutive AmpC was tested on agar containing oxacillin as an AmpC inhibitor. All producers were tested for antimicrobial susceptibility to amikacin, imipenem, meropenem, colisitin, piperacillin/tazobactam, cefepime, cefoperazone/sulbactam and tigecycline by the disk diffusion method. We also used modified Christensen method to find out the ability of isolates to produce biofilm. There were detected 88 (83.01 %) ESBL producers, 15 (14.15 %) constitutive AmpC producers, 2 (1.89 %) isolates producing both ESBL and constitutive AmpC and 1 (0.94 %) strain producing both ESBL and inducible AmpC. Eighty eight, i.e. 83.02 % of tested strains were susceptible to amikacin, 105 (99.06 %) to imipenem as well as to meropenem, 103 (97.17 %) to colistin, 43 ( 40.57 %) to piperacilllin/tazobactam, 82 (77.36 %) to cefepime and 49 (46.22 %) to cefoperazone/sulbactam. All the tested strains were susceptible to tigecycline. Biofilm was produced by 6 (5.66 %) strains.
ER  -

ČERNOHORSKÁ, Lenka, Zuzana VALIŠOVÁ, Vladana WOZNICOVÁ a David ŠMAJS. Characterization of uropathogenic E. coli strains with reduced susceptibility or resistance to extended spectrum cephalosporines. In \textit{Control and Mangement of Pathogenic Escherichia coli.}. 2009.

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