Analysis of T-rich oligonucleotides labelled with osmium
tetroxide  2,2´- bipyridine by capillary zone electrophoresis

D 2010

Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis

BITTOVÁ, Miroslava; Pavel KOSTEČKA; Libuše TRNKOVÁ a Miroslav FOJTA

Základní údaje

Originální název

Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis

Autoři

BITTOVÁ, Miroslava; Pavel KOSTEČKA; Libuše TRNKOVÁ a Miroslav FOJTA

Vydání

Olomouc, Acta Universitatis Palackianae Olomucensis Chemica 47S, od s. 78-79, 158 s. 2010

Nakladatel

Palacký University, Olomouc

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/10:00043430

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-244-2470-5

ISSN

Klíčová slova anglicky

oligonucleotides osmium tetroxide capillary electrophoresis

Změněno: 18. 1. 2013 19:49, Mgr. Miroslava Bittová, Ph.D.

Anotace

V originále

Labeling of nucleic acids offers broader option for its biochemical and physicochemical studies by the analytical separation methods such as capillary electrophoresis. Suitable labeling allows monitoring of DNA hybridization as well as following of DNA interactions with other biological and/or non-biological compounds. Chemical DNA probe consisting of osmium tetroxide and 2,2'-bipyridine (Os,bipy) is well-known especially for its electrochemical activity. In contrary to vanishing reactivity with purines, Os,bipy reagent creates covalent adducts with pyrimidine bases. The fastest adduct formation was observed namely with thymine. In our work, we analyzed selected pentanucleotides with various number and position of thymine bases and we monitored its reactivity with Os,bipy reagent. Our interest was also taken to ageing of Os,bipy reagent and the role of reagent abundance in mixture with oligonucleotide was studied. Capillary zone electrophoresis as an analytical technique was chosen for the sufficient ability to monitor the changes of the modified oligonucleotides.

Návaznosti

LC06035, projekt VaV

Název: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu

Citovat

BITTOVÁ, Miroslava; Pavel KOSTEČKA; Libuše TRNKOVÁ a Miroslav FOJTA. Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis. In Acta Universitatis Palackianae Olomucensis Chemica 47S. Olomouc: Palacký University, Olomouc, 2010, s. 78-79, 158 s. ISBN 978-80-244-2470-5.

@inproceedings{874334,
   author = {Bittová, Miroslava and Kostečka, Pavel and Trnková, Libuše and Fojta, Miroslav},
   address = {Olomouc},
   booktitle = {Acta Universitatis Palackianae Olomucensis Chemica 47S},
   keywords = {oligonucleotides osmium tetroxide capillary electrophoresis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Olomouc},
   isbn = {978-80-244-2470-5},
   pages = {78-79},
   publisher = {Palacký University, Olomouc},
   title = {Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis},
   year = {2010}
}

TY  - CONF
ID  - 874334
AU  - Bittová, Miroslava - Kostečka, Pavel - Trnková, Libuše - Fojta, Miroslav
PY  - 2010
TI  - Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis
PB  - Palacký University, Olomouc
CY  - Olomouc
SN  - 9788024424705
KW  - oligonucleotides osmium tetroxide capillary electrophoresis
N2  - Labeling of nucleic acids offers broader option for its biochemical and physicochemical studies by the analytical separation methods such as capillary electrophoresis. Suitable labeling allows monitoring of DNA hybridization as well as following of DNA interactions with other biological and/or non-biological compounds. Chemical DNA probe consisting of osmium tetroxide and 2,2'-bipyridine (Os,bipy) is well-known especially for its electrochemical activity. In contrary to vanishing reactivity with purines, Os,bipy reagent creates covalent adducts with pyrimidine bases. The fastest adduct formation was observed namely with thymine. In our work, we analyzed selected pentanucleotides with various number and position of thymine bases and we monitored its reactivity with Os,bipy reagent. Our interest was also taken to ageing of Os,bipy reagent and the role of reagent abundance in mixture with oligonucleotide was studied. Capillary zone electrophoresis as an analytical technique was chosen for the sufficient ability to monitor the changes of the modified oligonucleotides.
ER  -

BITTOVÁ, Miroslava; Pavel KOSTEČKA; Libuše TRNKOVÁ a Miroslav FOJTA. Analysis of T-rich oligonucleotides labelled with osmium tetroxide 2,2´- bipyridine by capillary zone electrophoresis. In \textit{Acta Universitatis Palackianae Olomucensis Chemica 47S}. Olomouc: Palacký University, Olomouc, 2010, s.~78-79, 158 s. ISBN~978-80-244-2470-5.

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