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@article{876077,
author = {Nováková, Dana and Pantůček, Roman and Hubálek, Zdeněk and Falsen, Enevold and Busse, HansandJuergen and Schumann, Peter and Sedláček, Ivo},
article_location = {Reading, United Kingdom},
article_number = {3},
keywords = {Staphylococcus microti; molecular taxonomy},
language = {eng},
issn = {1466-5026},
journal = {International Journal of Systematic and Evolutionary Microbiology},
title = {Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)},
url = {http://ijs.sgmjournals.org/cgi/content/abstract/60/3/566},
volume = {60},
year = {2010}
}
TY - JOUR
ID - 876077
AU - Nováková, Dana - Pantůček, Roman - Hubálek, Zdeněk - Falsen, Enevold - Busse, Hans-Juergen - Schumann, Peter - Sedláček, Ivo
PY - 2010
TI - Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis)
JF - International Journal of Systematic and Evolutionary Microbiology
VL - 60
IS - 3
SP - 566-573
EP - 566-573
PB - Society for General Microbiology
SN - 14665026
KW - Staphylococcus microti
KW - molecular taxonomy
UR - http://ijs.sgmjournals.org/cgi/content/abstract/60/3/566
N2 - Two strains of Gram-positive cocci were isolated from viscera of common voles (Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. Biochemical features and phylogenetic analysis based on 16S rRNA gene sequences showed that the strains are representatives of the genus Staphylococcus and assigned Staphylococcus muscae as the nearest relative. A detailed characterization done by ribotyping, rpoB and hsp60 gene sequencing, whole-cell protein analysis and rep-PCR using the (GTG)5 primer differentiated the two strains from all described staphylococci. DNA-DNA hybridization with the type strain of S. muscae demonstrated that the two strains should be considered as members of a novel species (26.8% reassociation). The two analysed strains were found to be coagulase-negative, novobiocin-susceptible, oxidase-negative cultures, phenotypically close to one another, but showing differences in ribotype profiles. The major fatty acids were iso-C15 : 0, iso-C17 : 0, anteiso- C15 : 0, C18 : 2v6,9c/anteiso-C18 : 0, C18 : 0 and C18 : 1v9c. MK-7 was the predominant isoprenoid quinone, with minor amounts of MK-6 and MK-8. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylglycerol and several unknown lipids. These results proved that the two isolates represent a novel staphylococcal species. The name proposed for this novel taxon is Staphylococcus microti sp. nov.; the type strain is 4005-LJ(m)T (5CCM 4903T 5CCUG 55861T 5DSM 22147T).
ER -
NOVÁKOVÁ, Dana, Roman PANTŮČEK, Zdeněk HUBÁLEK, Enevold FALSEN, Hans-Juergen BUSSE, Peter SCHUMANN a Ivo SEDLÁČEK. Staphylococcus microti sp. nov., isolated from the common vole (Microtus arvalis). \textit{International Journal of Systematic and Evolutionary Microbiology}. Reading, United Kingdom: Society for General Microbiology, roč.~60, č.~3, s.~566-573. ISSN~1466-5026. 2010.
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