The role of sterol-binding in elicitn interaction with tobacco

LOCHMAN, Jan, Jitka KLEMPOVÁ, Petra LITERÁKOVÁ, Nikola PTÁČKOVÁ and Tomáš KAŠPAROVSKÝ. The role of sterol-binding in elicitn interaction with tobacco. In FEBS Journal. 276th ed. Prague: Wiley-Blackwell, 2009, p. 241-242. ISSN 1742-464X.

Basic information

Original name

The role of sterol-binding in elicitn interaction with tobacco

Name in Czech

Role vazby sterolu při interakci elicitinů s tabákem

Authors

LOCHMAN, Jan (203 Czech Republic, guarantor), Jitka KLEMPOVÁ (203 Czech Republic), Petra LITERÁKOVÁ (203 Czech Republic), Nikola PTÁČKOVÁ (203 Czech Republic) and Tomáš KAŠPAROVSKÝ (203 Czech Republic)

Edition

276. vyd. Prague, FEBS Journal, p. 241-242, 2 pp. 2009

Publisher

Wiley-Blackwell

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.042

RIV identification code

RIV/00216224:14310/09:00029979

Organization unit

Faculty of Science

ISSN

UT WoS

000267069900720

Keywords (in Czech)

Elicitiny tabák rozpoznání

Keywords in English

Elicitins tobacco recognition

Tags

International impact

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Abstract

V originále

Cryptogein is a proteinaceous elicitor isolated from Phytophthora cryptogea able to trigger plant defence reaction. We prepared a series of cryptogein mutants which showed no-binding of sterol to study influence of sterol-binding for defence reaction activation. Cryptogein mutants were tested for induction of defence related genes expression covering the pathogenesis related proteins (PR1a (acidic), PR1b (basic), PR3Q) together with genes for epi-aristolochene synthase (EAS) and 9-lipooxygenase (LOX). Moreover, the accumulation of signalling compound salicylic acid (SA), phytoalexin capsidiol and malonyldialdehyde (MDA) as marker of cell death was measured. The mutants without sterol-binding activity showed different sensitivity to activate defence reaction in all tested markers; however mutant L15W/L36F demonstrated higher efficiency to trigger defence reaction. In addition, correlation of active oxygen species with EAS expression and capsidiol accumulation was observed.

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Links

GP203/09/P248, research and development project	Name: Mechanizmus interakce ergosterolu s rostlinnou buňkou Investor: Czech Science Foundation, Mechanism of ergosterol - plant cell interaction
MSM0021622413, plan (intention)	Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment