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@proceedings{877482,
author = {Hóbor, Fruzsina and Kubíček, Karel and Pasulka, Josef and Štefl, Richard},
booktitle = {Structure and RNA binding of Nab3},
keywords = {NMR; Nab3; RNA binding ; transcription termination; structure},
language = {eng},
title = {Structure and RNA binding of Nab3},
year = {2009}
}
TY - CONF
ID - 877482
AU - Hóbor, Fruzsina - Kubíček, Karel - Pasulka, Josef - Štefl, Richard
PY - 2009
TI - Structure and RNA binding of Nab3
KW - NMR
KW - Nab3
KW - RNA binding
KW - transcription termination
KW - structure
N2 - Besides mRNA, RNA polymerase II also transcribes a subset of small nuclear and small nucleoar RNAs, and a class of intergenic and anti-sense RNAs. Termination of these transcripts requires the nuclear pre-mRNA down- regulation (Nrd)1 and the nuclear polyadenylated RNA-binding (Nab)3 proteins, and the RNA helicase Sen1. In this so-called nonpoly(A) termination pathway, the RNA-binding proteins, Nrd1 and Nab3, recognize Nrd1- and Nab3-binding sites which is an initial step in the termination and subsequent processing or degradation of these transcripts. Recent studies identified sequence motives for Nrd1 and Nab3, as the signals that direct termination and exosome-TRAMP trimming/degradation of nonpolyadenylated transcripts. It was shown that the RNA-recognition motif (RRM) of Nrd1 and Nab3 bind to the GUA[A/G] and UCUU sequences, respectively. In addition, it was demonstrated that Nrd1 and Nab3 form a stable heterodimer and bind to snoRNA terminators that contain multiple Nrd1- and Nab3- binding sequences. To fully understand the structural basis behind the RNA recognition by the Nrd1-Nab3 complex, we use multidimensional NMR spectroscopy to determine the three- dimensional structures of the individual RRMs of Nrd1 and Nab3 and the minimal Nrd1- Nab3 heterodimer alone as well as in complex with their RNA substrates.
ER -
HÓBOR, Fruzsina, Karel KUBÍČEK, Josef PASULKA a Richard ŠTEFL. Structure and RNA binding of Nab3. Online. In \textit{Structure and RNA binding of Nab3}. 2009. ISSN~1211-5894. [citováno 2024-04-24]
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